Department of Respiratory Medicine, Taicang Hospital of Traditional Chinese Medicine, Suzhou, Jiangsu, 215400, P.R. China.
Department of Respiratory Medicine, Taicang Hospital of Traditional Chinese Medicine, Suzhou, Jiangsu, 215400, P. R. China.
Crit Rev Eukaryot Gene Expr. 2021;31(6):9-15. doi: 10.1615/CritRevEukaryotGeneExpr.2021039081.
PCAT29 has reported to exert tumor suppressive roles. In this study, we investigated the function of PCAT29 in non-small-cell lung cancer (NSCLC). Expression levels of PCAT29, miR-494, and PTEN in non-tumor and NSCLC tumor tissue samples were measured by performing quantitative reverse transcription polymerase chain reaction. Cell apoptosis and proliferation analyses were formed to study the effects of overexpression of PCAT29, miR-494, and PTEN on apoptosis and proliferation of H23 cells. It was observed that PCAT29 was down-regulated in NSCLC and predicted poor survival. In NSCLC cells, miR-494 was inversely, while PTEN was positively correlated with PCAT29. In NSCLC cells, overexpression of PCAT29 led to down-regulated miR-494 and up-regulated PTEN. Overexpression of miR-494 resulted in down-regulated PTEN but did not affect the expression of PCAT29. Overexpression of PTEN affected neither PCAT29 nor miR-494. In addition, overexpression of PCAT29 and PTEN resulted in increased apop-totic rate and decreased proliferation of NSCLC cells. miR-494 played an opposite role and attenuated the effects of overexpression of PCAT29. Therefore, PCAT29 may up-regulate PTEN by down-regulating miR-494 to suppress the progression of NSCLC cells.
PCAT29 已被报道发挥肿瘤抑制作用。在本研究中，我们研究了 PCAT29 在非小细胞肺癌（NSCLC）中的功能。通过定量逆转录聚合酶链反应测量非肿瘤和 NSCLC 肿瘤组织样本中 PCAT29、miR-494 和 PTEN 的表达水平。进行细胞凋亡和增殖分析，以研究过表达 PCAT29、miR-494 和 PTEN 对 H23 细胞凋亡和增殖的影响。结果观察到 PCAT29 在 NSCLC 中下调，并预测预后不良。在 NSCLC 细胞中，miR-494 与 PCAT29 呈负相关，而 PTEN 与 PCAT29 呈正相关。在 NSCLC 细胞中，过表达 PCAT29 导致 miR-494 下调和 PTEN 上调。过表达 miR-494 导致 PTEN 下调，但不影响 PCAT29 的表达。过表达 PTEN 既不影响 PCAT29 也不影响 miR-494。此外，过表达 PCAT29 和 PTEN 导致 NSCLC 细胞凋亡率增加和增殖减少。miR-494 发挥相反的作用，减弱了过表达 PCAT29 的作用。因此，PCAT29 可能通过下调 miR-494 来上调 PTEN，从而抑制 NSCLC 细胞的进展。
