lncRNA LANCL1-AS1的上调通过miR-3680-3p/GMFG轴抑制非小细胞肺癌的进展。

Upregulation of lncRNA LANCL1-AS1 inhibits the progression of non-small-cell lung cancer via the miR-3680-3p/GMFG axis.

作者信息

Pan Hui, Peng Jing, Qiao Xiaoni, Gao Han

机构信息

Department of Respiratory Medicine, Gansu Provincial Hospital, Lanzhou 730000, Gansu, China.

Department of Customer Service Center, Gansu Provincial Hospital, Lanzhou 730000, Gansu, China.

出版信息

Open Med (Wars). 2023 Mar 13;18(1):20230666. doi: 10.1515/med-2023-0666. eCollection 2023.

Patients with non-small-cell lung cancer (NSCLC) have a low survival rate. Long non-coding RNA (LncRNA) LANCL1 antisense RNA 1 (LANCL1-AS1) was indicated to be downregulated in NSCLC; however, its detailed function in NSCLC is unanswered. Real-time quantitative polymerase chain reaction revealed the downregulation of LANCL1-AS1 in NSCLC cell lines and subcellular fractionation assay showed that LANCL1-AS1 was mainly located in the cytoplasm of NSCLC cells. Cell counting kit-8, Transwell, and tube formation assays displayed that overexpression of LANCL1-AS1 suppressed NSCLC cell proliferation, migration, invasiveness, and angiogenesis . Animal experiments validated the tumor-suppressive role of LANCL1-AS1 in tumor-bearing mice. Mechanistically, LANCL1-AS1 upregulated glia maturation factor gamma (GMFG) expression by competitively binding to miR-3680-3p. GMFG knockdown reversed LANCL1-AS1 overexpression-mediated inhibitory impact on NSCLC malignant behaviors. Collectively, LANCL1-AS1 upregulation inhibits the progression of NSCLC by modulating the miR-3680-3p/GMFG axis.

非小细胞肺癌（NSCLC）患者的生存率较低。长链非编码RNA（LncRNA）LANCL1反义RNA 1（LANCL1-AS1）在NSCLC中呈下调表达；然而，其在NSCLC中的具体功能尚不清楚。实时定量聚合酶链反应显示NSCLC细胞系中LANCL1-AS1表达下调，亚细胞分级分离试验表明LANCL1-AS1主要位于NSCLC细胞的细胞质中。细胞计数试剂盒-8、Transwell和管形成试验显示，LANCL1-AS1过表达抑制NSCLC细胞增殖、迁移、侵袭和血管生成。动物实验验证了LANCL1-AS1在荷瘤小鼠中的肿瘤抑制作用。机制上，LANCL1-AS1通过竞争性结合miR-3680-3p上调胶质细胞成熟因子γ（GMFG）的表达。GMFG基因敲低逆转了LANCL1-AS1过表达介导的对NSCLC恶性行为的抑制作用。总之，LANCL1-AS1的上调通过调节miR-3680-3p/GMFG轴抑制NSCLC的进展。