NrtA 的生物物理特性与表征：一种基于蛋白质的水合硝酸盐和亚硝酸盐识别宿主。
Biophysical and characterization of NrtA: a protein-based host for aqueous nitrate and nitrite recognition.
机构信息
Department of Chemistry and Biochemistry, The University of Texas at Dallas, Richardson, TX 75080, USA.
Department of Biological Sciences, The University of Texas at Dallas, Richardson, Texas 75080, USA.
出版信息
Chem Commun (Camb). 2022 Jan 20;58(7):965-968. doi: 10.1039/d1cc05879g.
Abstract
Nitrate and nitrite are key components of the global nitrogen cycle. As such, Nature has evolved proteins as biological supramolecular hosts for the recognition, translocation, and transformation of both nitrate and nitrite. To understand the supramolecular principles that govern these anion-protein interactions, here, we employ a hybrid biophysical and approach to characterize the thermodynamic properties and protein dynamics of NrtA from the cyanobacterium for the recognition of nitrate and nitrite.
摘要
硝酸盐和亚硝酸盐是全球氮循环的关键组成部分。因此，自然界进化出了蛋白质作为生物超分子主体，用于识别、转运和转化硝酸盐和亚硝酸盐。为了了解这些阴离子-蛋白质相互作用的超分子原理，在这里，我们采用混合生物物理和 方法来表征蓝藻 NrtA 识别硝酸盐和亚硝酸盐的热力学性质和蛋白质动力学。
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