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1
Intrinsic versus extrinsic coagulation. Kinetic considerations.内源性凝血与外源性凝血。动力学考量。
Biochem J. 1986 Nov 1;239(3):757-62. doi: 10.1042/bj2390757.
2
Reactivity of bovine blood coagulation factor IXa beta, factor Xa beta, and factor XIa toward fluorogenic peptides containing the activation site sequences of bovine factor IX and factor X.牛凝血因子IXaβ、因子Xaβ和因子XIa对含有牛因子IX和因子X激活位点序列的荧光肽的反应性。
Biochemistry. 1983 Mar 1;22(5):1021-9. doi: 10.1021/bi00274a004.
3
Kinetics of the Factor XIa catalyzed activation of human blood coagulation Factor IX.凝血因子XIa催化人凝血因子IX活化的动力学
J Clin Invest. 1984 May;73(5):1392-9. doi: 10.1172/JCI111343.
4
Assembly and expression of an intrinsic factor IX activator complex on the surface of cultured human endothelial cells.在培养的人内皮细胞表面组装并表达内因子IX激活剂复合物。
J Biol Chem. 1992 Oct 5;267(28):19833-9.
5
Mapping the active sites of bovine thrombin, factor IXa, factor Xa, factor XIa, factor XIIa, plasma kallikrein, and trypsin with amino acid and peptide thioesters: development of new sensitive substrates.利用氨基酸和肽硫酯对牛凝血酶、因子IXa、因子Xa、因子XIa、因子XIIa、血浆激肽释放酶和胰蛋白酶的活性位点进行定位：新型敏感底物的开发
Biochemistry. 1981 Dec 8;20(25):7196-206. doi: 10.1021/bi00528a022.
6
Functional characterization of platelet-bound factor XIa: retention of factor XIa activity on the platelet surface.血小板结合因子XIa的功能特性：因子XIa活性在血小板表面的保留
Blood. 1986 Jul;68(1):225-30.
7
Assembly of the intrinsic factor X activating complex--interactions between factor IXa, factor VIIIa and phospholipid.内源性因子X激活复合物的组装——因子IXa、因子VIIIa与磷脂之间的相互作用
Thromb Haemost. 1985 Jun 24;53(3):396-400.
8
Evidence that plasma lipoproteins inhibit the factor VIIa-tissue factor complex by a different mechanism that extrinsic pathway inhibitor.
Blood. 1987 Dec;70(6):1947-54.
9
The effect of platelets in the activation of human blood coagulation factor IX by factor XIa.血小板在因子XIa激活人凝血因子IX中的作用。
Blood. 1986 Jul;68(1):140-8.
10
Extrinsic activation of human coagulation factors IX and X on the endothelial surface.人凝血因子IX和X在内皮表面的外源性激活。
Thromb Haemost. 1991 Sep 2;66(3):283-91.

引用本文的文献

1
Exactin: A specific inhibitor of Factor X activation by extrinsic tenase complex from the venom of Hemachatus haemachatus.海蛇凝血酶原激活物：从矛头蝮蛇蛇毒中提取的外切Tenase 复合物对因子 X 激活的特异性抑制剂。
Sci Rep. 2016 Aug 25;6:32036. doi: 10.1038/srep32036.
2
Thrombin activity propagates in space during blood coagulation as an excitation wave.凝血过程中，凝血酶活性在空间中作为激发波传播。
Biophys J. 2012 Nov 21;103(10):2233-40. doi: 10.1016/j.bpj.2012.10.011. Epub 2012 Nov 20.
3
Inhibitor of the factor VIIa-tissue factor complex is reduced in patients with disseminated intravascular coagulation but not in patients with severe hepatocellular disease.弥散性血管内凝血患者体内因子VIIa - 组织因子复合物的抑制剂水平降低，但严重肝细胞疾病患者体内该抑制剂水平未降低。
J Clin Invest. 1987 Jun;79(6):1874-8. doi: 10.1172/JCI113030.
4
The contributions of Ca2+, phospholipids and tissue-factor apoprotein to the activation of human blood-coagulation factor X by activated factor VII.钙离子、磷脂和组织因子载脂蛋白对活化的因子 VII 激活人凝血因子 X 的作用。
Biochem J. 1990 Jan 15;265(2):327-36. doi: 10.1042/bj2650327.
5
Replacement of isoleucine-397 by threonine in the clotting proteinase factor IXa (Los Angeles and Long Beach variants) affects macromolecular catalysis but not L-tosylarginine methyl ester hydrolysis. Lack of correlation between the ox brain prothrombin time and the mutation site in the variant proteins.凝血蛋白酶因子IXa中异亮氨酸-397被苏氨酸取代（洛杉矶和长滩变体）会影响大分子催化，但不影响L-甲苯磺酰精氨酸甲酯水解。牛脑凝血酶原时间与变体蛋白中突变位点之间缺乏相关性。
Biochem J. 1990 Jan 1;265(1):219-25. doi: 10.1042/bj2650219.

本文引用的文献

1
Kinetics of factor IX activation via the extrinsic pathway. Dependence of Km on tissue factor.通过外源性途径激活因子IX的动力学。米氏常数（Km）对组织因子的依赖性。
J Biol Chem. 1980 Jun 25;255(12):5703-7.
2
A simplified procedure for purification of human prothrombin, factor IX and factor X.一种纯化人凝血酶原、因子IX和因子X的简化程序。
Prep Biochem. 1981;11(4):397-412. doi: 10.1080/00327488108065531.
3
Inactivation of factor XIa by plasma protease inhibitors: predominant role of alpha 1-protease inhibitor and protective effect of high molecular weight kininogen.血浆蛋白酶抑制剂对因子XIa的灭活作用：α1-蛋白酶抑制剂的主要作用及高分子量激肽原的保护作用
J Clin Invest. 1982 Apr;69(4):844-52. doi: 10.1172/jci110524.
4
Blood coagulation.血液凝固
Annu Rev Biochem. 1980;49:765-811. doi: 10.1146/annurev.bi.49.070180.004001.
5
Binding of human factor VII and VIIa to monocytes.人凝血因子VII和VIIa与单核细胞的结合。
J Clin Invest. 1982 Sep;70(3):526-35. doi: 10.1172/jci110644.
6
Cooperative Ca2+ binding to human factor IX. Effects of Ca2+ on the kinetic parameters of the activation of factor IX by factor XIa.钙离子与人凝血因子IX的协同结合。钙离子对凝血因子XIa激活凝血因子IX动力学参数的影响。
J Biol Chem. 1982 Apr 25;257(8):4127-32.
7
Human factor XII (Hageman factor).人凝血因子 XII（哈格曼因子）。
Methods Enzymol. 1981;80 Pt C:198-211. doi: 10.1016/s0076-6879(81)80018-3.
8
Complete amino acid sequence of the light chain of human blood coagulation factor X: evidence for identification of residue 63 as beta-hydroxyaspartic acid.人凝血因子X轻链的完整氨基酸序列：将第63位残基鉴定为β-羟基天冬氨酸的证据
Biochemistry. 1983 Jun 7;22(12):2875-84. doi: 10.1021/bi00281a016.
9
Role of human factor VIII in factor X activation.人凝血因子VIII在凝血因子X激活中的作用。
J Clin Invest. 1982 Apr;69(4):950-8. doi: 10.1172/jci110534.
10
Purification and characterization of bovine tissue factor.牛组织因子的纯化与特性分析
J Biol Chem. 1981 Aug 25;256(16):8324-31.

内源性凝血与外源性凝血。动力学考量。

Intrinsic versus extrinsic coagulation. Kinetic considerations.

作者信息

Warn-Cramer B J, Bajaj S P

出版信息

Biochem J. 1986 Nov 1;239(3):757-62. doi: 10.1042/bj2390757.

DOI:10.1042/bj2390757
PMID:3493767
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1147351/
Abstract

A study to compare the kinetics of activation of factor IX by Factor XIa/Ca2+ and by Factor VIIa/tissue factor/Ca2+ has been undertaken. When purified human proteins, detergent-extracted brain tissue factor and tritiated-activation-peptide-release assays were utilized, the kinetic constants obtained were: Km = 310 nM, kcat. = 25 min-1 for Factor XIa and Km = 210 nM, kcat. = 15 min-1 for Factor VIIa. The kinetic constants for the activation of Factor X by Factor VIIa/brain tissue factor were: Km = 205 nM, kcat. = 70 min-1. Predicted rates for the generation of Factor IXa and Factor Xa were obtained when human monocytic tumour U937 cells (source of tissue factor) and Factor VIIa were used to form the activator. In other experiments, inclusion of high-Mr kininogen did not increase the activation rates of Factor IX by Factor XIa in the presence or absence of platelets and/or denuded rabbit aorta. These kinetic data strongly indicate that both Factor XIa and Factor VIIa play physiologically significant roles in the activation of Factor IX.

摘要

已开展一项研究，以比较因子XIa/Ca2+和因子VIIa/组织因子/Ca2+对因子IX的激活动力学。当使用纯化的人蛋白、经去污剂提取的脑组织因子和氚标记的激活肽释放测定法时，获得的动力学常数为：因子XIa的Km = 310 nM，kcat. = 25分钟-1；因子VIIa的Km = 210 nM，kcat. = 15分钟-1。因子VIIa/脑组织因子对因子X的激活动力学常数为：Km = 205 nM，kcat. = 70分钟-1。当使用人单核细胞肿瘤U937细胞（组织因子来源）和因子VIIa形成激活剂时，获得了因子IXa和因子Xa生成的预测速率。在其他实验中，无论有无血小板和/或去内皮兔主动脉，加入高分子量激肽原均未提高因子XIa对因子IX的激活速率。这些动力学数据有力地表明，因子XIa和因子VIIa在因子IX的激活中均发挥着生理上重要的作用。