光刺激鱿鱼视网膜中肌醇三磷酸的快速形成。
Light stimulates the rapid formation of inositol trisphosphate in squid retinas.
作者信息
Szuts E Z, Wood S F, Reid M S, Fein A
出版信息
Biochem J. 1986 Dec 15;240(3):929-32. doi: 10.1042/bj2400929.
Abstract
To test the hypothesis that inositol trisphosphate (InsP3) mediates adaptation and excitation in invertebrate photoreceptors, we measured its formation on a rapid time scale in squid retinas. For squid, excitation and adaption occurs within 0.1 and 1-2 s respectively. We could detect an elevation in InsP3 within 200 ms of a bright flash. This increase is about 240% over dark basal levels and is maintained for at least 2 min after a flash. The increase probably occurs in the photoreceptors, which are the only neurons in squid retinas. Analysis by h.p.l.c. indicates that the light-regulated isomer is Ins(1,4,5)P3, which is formed by the hydrolysis of phosphatidylinositol bisphosphate (PtdInsP2).
摘要
为了验证肌醇三磷酸（InsP3）介导无脊椎动物光感受器的适应性和兴奋性这一假设，我们在乌贼视网膜中以快速时间尺度测量了其形成过程。对于乌贼而言，兴奋和适应分别在0.1秒和1 - 2秒内发生。在明亮闪光后200毫秒内，我们能够检测到InsP3的升高。这种增加比黑暗基础水平高出约240%，并且在闪光后至少维持2分钟。这种增加可能发生在光感受器中，光感受器是乌贼视网膜中唯一的神经元。高效液相色谱分析表明，光调节的异构体是Ins(1,4,5)P3，它由磷脂酰肌醇二磷酸（PtdInsP2）水解形成。
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本文引用的文献
1
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Nature. 1962 Jun 2;194:844-7. doi: 10.1038/194844a0.
2
myo-Inositol polyphosphate may be a messenger for visual excitation in Limulus photoreceptors.肌醇多磷酸可能是鲎光感受器中视觉兴奋的信使。
Nature. 1984;311(5982):160-3. doi: 10.1038/311160a0.
3
Photoreceptor excitation and adaptation by inositol 1,4,5-trisphosphate.肌醇1,4,5 - 三磷酸介导的光感受器兴奋与适应
Nature. 1984;311(5982):157-60. doi: 10.1038/311157a0.
4
The structure of cytoplasm in directly frozen cultured cells. I. Filamentous meshworks and the cytoplasmic ground substance.直接冷冻培养细胞中细胞质的结构。I. 丝状网络和细胞质基质。
J Cell Biol. 1984 Nov;99(5):1655-68. doi: 10.1083/jcb.99.5.1655.
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Biochem Biophys Res Commun. 1984 Dec 28;125(3):1137-42. doi: 10.1016/0006-291x(84)91402-5.
6
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J Biol Chem. 1983 Feb 25;258(4):2076-8.
7
The polyphosphoinositide phosphodiesterase of erythrocyte membranes.红细胞膜的多磷酸肌醇磷酸二酯酶
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Biochemistry. 1984 May 22;23(11):2347-52. doi: 10.1021/bi00306a004.
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