Fein A, Payne R, Corson D W, Berridge M J, Irvine R F
Nature. 1984;311(5982):157-60. doi: 10.1038/311157a0.
A central question concerning vision is the identity of the biochemical pathway that underlies phototransduction. The large size of the ventral photoreceptors of Limulus polyphemus renders them a favourite preparation for investigating this problem. The fact that a single photon opens approximately 1,000 ionic channels in these photoreceptors suggests the need for an internal transmitter. We have investigated whether inositol 1,4,5-trisphosphate (InsP3) functions as such an internal transmitter, given that InsP3 may act as an intracellular messenger in other cellular processes. Here we report that in Limulus, intracellular pressure injection of InsP3 both excites and adapts ventral photoreceptors in a manner similar to light.
一个与视觉相关的核心问题是光转导背后的生化途径的特性。美洲鲎腹侧光感受器的较大尺寸使其成为研究该问题的理想标本。在这些光感受器中，单个光子可打开约1000个离子通道，这一事实表明需要一种内部递质。鉴于肌醇1,4,5 -三磷酸（InsP3）可能在其他细胞过程中充当细胞内信使，我们研究了InsP3是否充当这样一种内部递质。在此我们报告，在美洲鲎中，向细胞内压力注射InsP3会以类似于光的方式使腹侧光感受器兴奋并产生适应性变化。
