Biosensors Analysis Environment Laboratory, Université de Perpignan Via Domitia, F-66860 Perpignan, France.
Biodiversity and Microbial Biotechnologies Laboratory, USR 3579 Sorbonne Universités (UPMC), F-66650 Banyuls-sur-Mer, France.
Biosensors (Basel). 2021 Dec 13;11(12):513. doi: 10.3390/bios11120513.
Chlortoluron (3-(3-chloro-p-tolyl)-1,1-dimethyl urea) is an herbicide widely used in substitution to isoproturon to control grass weed in wheat and barley crops. Chlortoluron has been detected in groundwaters for more than 20 years; and dramatic increases in concentrations are observed after intense rain outbreaks. In this context; we developed an immunosensor for the determination of chlortoluron based on competitive binding of specific monoclonal antibodies on chlortoluron and immobilized biotinylated chlortoluron; followed by electrochemical detection on screen-printed carbon electrodes. The optimized immunosensor exhibited a logarithmic response in the range 0.01-10 µg·L; with a calculated detection limit (LOD) of 22.4 ng·L; which is below the maximum levels allowed by the legislation (0.1 µg·L). The immunosensor was used for the determination of chlortoluron in natural groundwaters, showing the absence of matrix effects.
绿麦隆（3-（3-氯-对甲苯基）-1,1-二甲基脲）是一种广泛用于替代异丙隆的除草剂，用于控制小麦和大麦作物中的杂草。绿麦隆在地下水中已经存在了 20 多年；在强降雨爆发后，其浓度会急剧增加。在这种情况下；我们开发了一种基于绿麦隆特异性单克隆抗体与固定化生物素化绿麦隆的竞争结合的绿麦隆测定免疫传感器，随后在丝网印刷碳电极上进行电化学检测。优化后的免疫传感器在 0.01-10 µg·L 的范围内呈对数响应，计算出的检测限（LOD）为 22.4 ng·L，低于法规允许的最高水平（0.1 µg·L）。该免疫传感器用于测定天然地下水中的绿麦隆，显示无基质效应。
