Kennedy K A, Wilton P, Mellander M, Rojas J, Sundell H
J Dev Physiol. 1986 Dec;8(6):421-33.
Fetal lung liquid secretion depends on active transport of chloride ions. Chloride secretion in the stomach is inhibited by epidermal growth factor (EGF). For this reason, the effect of EGF on lung liquid secretion was measured using the impermeant-tracer technique in chronically-prepared fetal sheep. Infusion of EGF over 4 h resulted in decreased lung liquid secretion (from 4.2 +/- 0.6 to 1.7 +/- 0.8 ml/h, P = 0.02) and significant dose related tachycardia. During the infusion, plasma epinephrine levels increased from 27 +/- 5 to 67 +/- 13 pg/ml (P = 0.05) and norepinephrine levels increased from 257 +/- 31 to 544 +/- 69 pg/ml (P = 0.01). Since it is known that beta-adrenergic agonists inhibit lung liquid secretion, subsequent studies were performed with beta-adrenergic blockade using propranolol. Infusion of EGF and propranolol resulted in a significant decrease in lung liquid secretion (from 8.9 +/- 2.1 to 3.0 +/- 1.1 ml/h, P = 0.03). Infusion of propranolol alone had no demonstrable effect on lung liquid secretion. It is concluded that acute EGF infusion increases heart rate and stimulates catecholamine secretion in fetal sheep. EGF also inhibits lung liquid secretion, an effect which appears to be independent of a possible indirect catecholamine effect.
胎儿肺液分泌依赖于氯离子的主动转运。胃中的氯离子分泌受到表皮生长因子（EGF）的抑制。因此，采用不透过性示踪技术，在长期制备的胎羊中测量了EGF对肺液分泌的影响。4小时内输注EGF导致肺液分泌减少（从4.2±0.6降至1.7±0.8毫升/小时，P = 0.02），且出现显著的剂量相关心动过速。在输注过程中，血浆肾上腺素水平从27±5升至67±13皮克/毫升（P = 0.05），去甲肾上腺素水平从257±31升至544±69皮克/毫升（P = 0.01）。由于已知β-肾上腺素能激动剂会抑制肺液分泌，随后使用普萘洛尔进行β-肾上腺素能阻滞研究。输注EGF和普萘洛尔导致肺液分泌显著减少（从8.9±2.1降至3.0±1.1毫升/小时，P = 0.03）。单独输注普萘洛尔对肺液分泌没有明显影响。结论是，急性输注EGF可增加胎羊心率并刺激儿茶酚胺分泌。EGF还抑制肺液分泌，这种作用似乎独立于可能的间接儿茶酚胺效应。
