Structure of the Cell-Wall-Associated Polysaccharides from the Deep-Sea Marine Bacterium KMM 9415.

Two cell-wall-associated polysaccharides were isolated and purified from the deep-sea marine bacterium KMM 9415, purified by ultracentrifugation and enzymatic treatment, separated by chromatographic techniques, and studied by sugar analyses and NMR spectroscopy. The first polysaccharide with a molecular weight of about 20.7 kDa was found to contain d-arabinose, and the following structure of its disaccharide repeating unit was established: →2)-α-d-Ara-(1→5)-α-d-Ara-(1→. The second polysaccharide was shown to consist of d-galactose and a rare component of bacterial glycans-d-xylulose: →3)-α-d-Gal-(1→3)-β-d-Xlu-(1→.