Loo D T, Fuquay J I, Rawson C L, Barnes D W
Science. 1987 Apr 10;236(4798):200-2. doi: 10.1126/science.3494308.
Mouse embryo cells cultured in vitro in serum-supplemented media undergo growth crisis, resulting in the loss of genomically normal cells prior to the appearance of established, aneuploid cell lines. Mouse embryo cells established and maintained for multiple passages in the absence of serum did not exhibit growth crisis or gross chromosomal aberration. Cells cultured under these conditions were dependent on epidermal growth factor for survival. Proliferation was reversibly inhibited by serum or platelet-free plasma, suggesting that mouse embryo cultures maintained by conventional procedures are under the influence of inhibitory factors.
在补充血清的培养基中体外培养的小鼠胚胎细胞会经历生长危机，导致在建立非整倍体细胞系之前基因组正常的细胞丢失。在无血清条件下建立并传代多次的小鼠胚胎细胞未表现出生长危机或明显的染色体畸变。在这些条件下培养的细胞依赖表皮生长因子存活。血清或无血小板血浆可可逆地抑制细胞增殖，这表明通过传统方法维持的小鼠胚胎培养物受到抑制因子的影响。
