Oh Tong In, Kang Min Ji, Jeong You Jeong, Zhang Tingting, Yeo Seung Geun, Park Dong Choon
Department of Biomedical Engineering, College of Medicine, Kyung Hee University, Seoul 02447, Korea.
Medical Science Research Institute, Kyung Hee University Medical Center, Seoul 02447, Korea.
Diagnostics (Basel). 2021 Dec 14;11(12):2354. doi: 10.3390/diagnostics11122354.
The successful management of cervical intraepithelial neoplasia (CIN) with proper screening and treatment methods could prevent cervical cancer progression. We propose a bioimpedance spectroscopic measurement device and a multi-electrode probe as an independent screening tool for CIN. To evaluate the performance of this screening method, we enrolled 123 patients, including 69 patients with suspected CIN and 54 control patients without cervical dysplasia who underwent a hysterectomy for benign disease (non-CIN). Following conization, the electrical properties of the excised cervical tissue were characterized using an electrical bioimpedance spectroscopy-based multi-electrode probe. Twenty-eight multifrequency voltages were collected through the two concentric array electrodes via a sensitivity-optimized measurement protocol based on an electrical energy concentration method. The electrical properties of the CIN and non-CIN groups were compared with the results of the pathology reports. Reconstructed resistivity tended to decrease in the CIN and non-CIN groups as frequency increased. Reconstructed resistivity from 625 Hz to 50 kHz differed significantly between the CIN and non-CIN groups ( < 0.001). Using 100 kHz as the reference, the difference between the CIN and non-CIN groups was significant. Based on the difference in reconstructed resistivity between 100 kHz and the other frequencies, this method had a sensitivity of 94.3%, a specificity of 84%, and an accuracy of 90% in CIN screening. The feasibility of noninvasive CIN screening was confirmed through the difference in the frequency spectra evaluated in the excised tissue using the electrical bioimpedance spectroscopy-based multi-electrode screening probe.
采用适当的筛查和治疗方法成功管理宫颈上皮内瘤变（CIN）可预防宫颈癌进展。我们提出一种生物阻抗光谱测量装置和多电极探头作为CIN的独立筛查工具。为评估这种筛查方法的性能，我们招募了123名患者，包括69名疑似CIN患者和54名因良性疾病（非CIN）接受子宫切除术的无宫颈发育异常的对照患者。锥切术后，使用基于生物电阻抗光谱的多电极探头对切除的宫颈组织的电学特性进行表征。通过基于电能集中法的灵敏度优化测量方案，通过两个同心阵列电极收集28个多频电压。将CIN组和非CIN组的电学特性与病理报告结果进行比较。随着频率增加，CIN组和非CIN组的重建电阻率均呈下降趋势。CIN组和非CIN组在625 Hz至50 kHz的重建电阻率差异显著（<0.001）。以100 kHz作为参考，CIN组和非CIN组之间的差异显著。基于100 kHz与其他频率之间重建电阻率的差异，该方法在CIN筛查中的灵敏度为94.3%，特异性为84%，准确率为90%。通过使用基于生物电阻抗光谱的多电极筛查探头评估切除组织中的频谱差异，证实了无创CIN筛查的可行性。
