Wysokińska Anna, Szablicka Dorota
Faculty of Agrobioengineering and Animal Husbandry, Siedlce University of Natural Sciences and Humanities, 08110 Siedlce, Poland.
Animals (Basel). 2021 Nov 25;11(12):3373. doi: 10.3390/ani11123373.
The aim of the study was to assess changes in the integrity of sperm cell membranes during the storage of semen collected from Duroc × Pietrain crossbred boars and purebred boars of the component breeds. To compare the cell membrane integrity of sperm heads in crossbred and purebred boars, heterosis effects were estimated. The study was conducted on 48 ejaculates collected from Duroc × Pietrain crossbred boars and from purebred Duroc and Pietrain boars used for artificial insemination. Microscope slides were prepared from each ejaculate for the evaluation of the cell membrane integrity of the sperm, at 1, 24, 48, 72, and 96 h after collection of the ejaculate. Diluted ejaculates were stored at 17 °C. Sperm membrane integrity was analysed by two methods: SYBR-14/PI and eosin-nigrosin. Our results showed that the cell membrane integrity of sperm heads changed with storage time, but the extent of the changes varied depending on the genetic group of boars. The semen of Duroc × Pietrain crossbreds was clearly seen to be less sensitive to storage conditions than that of boars of the parent breeds, which was confirmed by the calculated heterosis effects. The percentage of sperm with an intact cell membrane was higher in crossbred boars than in purebred boars ( ≤ 0.05). In addition, significantly fewer moribund sperm spermatozoa and spermatozoa with a damaged cell membrane were observed in crossbred boars ( ≤ 0.05). In the semen of purebred Duroc and Pietrain boars, the cell membrane integrity of the sperm should be assessed more often during storage than in the semen of Duroc × Pietrain crossbred boars. This study provides valuable information for the development and implementation of semen quality monitoring in crossbred boars and boars of the parent breeds during storage at 17 °C with respect to the cell membrane structure of sperm heads. The evaluation methods used effectively identify damage to the cell membranes of the sperm during semen storage.
本研究的目的是评估从杜洛克×皮特兰杂交公猪和组成品种的纯种公猪采集的精液在储存期间精子细胞膜完整性的变化。为了比较杂交公猪和纯种公猪精子头部的细胞膜完整性，估计了杂种优势效应。本研究使用了48份从用于人工授精的杜洛克×皮特兰杂交公猪以及纯种杜洛克和皮特兰公猪采集的精液样本。从每份精液样本制备显微镜载玻片，用于在精液采集后1、24、48、72和96小时评估精子的细胞膜完整性。稀释后的精液样本储存在17°C环境中。通过两种方法分析精子膜完整性：SYBR-14/PI法和伊红-黑色素法。我们的结果表明，精子头部的细胞膜完整性随储存时间而变化，但变化程度因公猪的遗传群体而异。杜洛克×皮特兰杂交种的精液对储存条件的敏感性明显低于亲本品种公猪的精液，计算得出的杂种优势效应证实了这一点。杂交公猪中细胞膜完整的精子百分比高于纯种公猪（P≤0.05）。此外，在杂交公猪中观察到的濒死精子和细胞膜受损的精子明显更少（P≤0.05）。在纯种杜洛克和皮特兰公猪的精液中，储存期间应比杜洛克×皮特兰杂交公猪的精液更频繁地评估精子的细胞膜完整性。本研究为在17°C储存期间监测杂交公猪和亲本品种公猪精液质量时精子头部细胞膜结构的发育和实施提供了有价值的信息。所使用的评估方法有效地识别了精液储存期间精子细胞膜的损伤。
