Schulze M, Rüdiger K, Waberski D
Institute for the Reproduction of Farm Animals Schönow Inc., Bernau, Germany.
Unit for Reproductive Medicine of Clinics/Clinic for Pigs and Small Ruminants, University of Veterinary Medicine Hannover, Hannover, Germany.
Reprod Domest Anim. 2015 Aug;50(4):684-7. doi: 10.1111/rda.12532. Epub 2015 May 13.
It is common practice to rotate boar semen doses during storage for prevention of sperm sedimentation. In this study, the effect of rotation of boar semen doses during storage on sperm quality was investigated. Manual turning twice daily and automatic rotation five times per hour resulted in the following effects: alkalinization of the BTS-extender, loss of membrane integrity at day 3, and loss of motility and changes in sperm kinematics during a thermoresistance test at day 5. Using a pH-stabilized variant of BTS extender, sperm motility and velocity decreased in continuously rotated samples, whereas membrane integrity and mitochondrial activity remain unaffected. It is concluded that rotation of semen samples adversely affects sperm quality and, therefore, should no longer be recommended for AI practice.
在精液储存期间旋转公猪精液剂量以防止精子沉降是常见做法。在本研究中，研究了储存期间公猪精液剂量旋转对精子质量的影响。每天手动翻转两次和每小时自动旋转五次产生了以下影响：BTS稀释液碱化、第3天膜完整性丧失、第5天热抗性试验期间活力丧失和精子运动学变化。使用pH稳定的BTS稀释液变体，持续旋转的样本中精子活力和速度降低，而膜完整性和线粒体活性未受影响。得出的结论是，精液样本的旋转会对精子质量产生不利影响，因此，不应再推荐用于人工授精实践。
