Li Xinfu, Zhou Shen, Zhang Jing, Zhou Zhihao, Xiong Qiang
College of Food Science and Light Industry, Nanjing Tech University, Nanjing 211800, China.
College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing 211800, China.
Animals (Basel). 2021 Dec 6;11(12):3475. doi: 10.3390/ani11123475.
Black soldier fly (BSF) larvae, (Diptera: Stratiomyidae) have emerged as an efficient system for the bioconversion of organic waste. Intestinal microorganisms are involved in several insect functions, including the development, nutrition, and physiology of the host. In order to transform the intestinal bacterial community of BSF directionally, six different potential functional strains (, , , , , and ) were added to aseptic food waste, and aseptic food waste was used without inoculants as a blank control to evaluate the changes in the intestinal microbiota of BSF under artificial intervention conditions. These six strains (which were isolated from the larval intestinal tract in selective media and then identified and screened) may be considered responsible for the functional characteristics of larvae. The results imply that the increase in the abundance of in the experimental group that was exposed to was significantly different to the other groups ( < 0.05). The results revealed that it is feasible to transform the intestinal microbiota of BSF directionally; there are differences in the proliferation of different strains in the intestine of BSF.
黑水虻（双翅目：水虻科）已成为一种高效的有机废物生物转化系统。肠道微生物参与多种昆虫功能，包括宿主的发育、营养和生理过程。为了定向改造黑水虻的肠道细菌群落，将六种不同的潜在功能菌株（、、、、和）添加到无菌食物垃圾中，并将未接种菌剂的无菌食物垃圾用作空白对照，以评估人工干预条件下黑水虻肠道微生物群的变化。这六种菌株（从选择性培养基中的幼虫肠道分离出来后进行鉴定和筛选）可能被认为对幼虫的功能特性负责。结果表明，暴露于的实验组中丰度增加，与其他组相比差异显著（<0.05）。结果表明定向改造黑水虻肠道微生物群是可行的；不同菌株在黑水虻肠道中的增殖存在差异。
