Asai R, Nakao I, Ito K, Ohashi Y, Saito T, Kataoka T
Gan To Kagaku Ryoho. 1987 Mar;14(3 Pt 2):922-30.
It is crucial to define the immunological characteristics of peripheral blood mononuclear cells in order to clarify the physiological state of cancer patients. In this study, we examined prostaglandin E2 (PGE2) and interleukin-1 (IL-1) production by plastic-adherent cells stimulated by lipopolysaccharide (LPS). The secretion of PGE2 and IL-1 into media depended on the dose of LPS. Although the addition of silica resulted in suppression of LPS-induced PGE2 production, it caused augmentation of IL-1 production. The effect of BRM therapy on IL-1 production was evaluated in five cancer patients. The results demonstrated that BRM therapy increased the levels of IL-1 at the late assessment point for 3 patients and their quality of life was improved. These findings suggest that production of IL-1 may be used as a monitor for the effectiveness of biotherapy. The association of PGE2 production with host antitumor response was evaluated in IFN-gamma therapy. The results showed that the PGE2 production ratio increased early in the therapy period and declined gradually, whereas the expression of HLA-DR antigen on monocytes and the level of IL-1 increased during the treatment. The exact mechanism by which BRM activates monocytes is unknown. It is possible that a distinct subpopulation of monocytes is responsible for this effect.
为阐明癌症患者的生理状态，定义外周血单个核细胞的免疫学特征至关重要。在本研究中，我们检测了脂多糖（LPS）刺激的贴壁细胞产生前列腺素E2（PGE2）和白细胞介素-1（IL-1）的情况。PGE2和IL-1向培养基中的分泌取决于LPS的剂量。虽然添加二氧化硅会抑制LPS诱导的PGE2产生，但会导致IL-1产生增加。对5名癌症患者评估了生物反应调节剂（BRM）疗法对IL-1产生的影响。结果表明，BRM疗法使3名患者在后期评估点的IL-1水平升高，且他们的生活质量得到改善。这些发现表明，IL-1的产生可用作生物疗法有效性的监测指标。在干扰素-γ疗法中评估了PGE2产生与宿主抗肿瘤反应的关联。结果显示，治疗初期PGE2产生率升高，随后逐渐下降，而治疗期间单核细胞上HLA-DR抗原的表达及IL-1水平升高。BRM激活单核细胞的确切机制尚不清楚。可能是单核细胞的一个独特亚群负责此效应。
