VA San Diego Healthcare System, La Jolla, CA 92161, USA.
Division of Surgical Oncology, Department of Surgery, University of California San Diego, La Jolla, CA 92037, USA.
Biomolecules. 2021 Dec 2;11(12):1819. doi: 10.3390/biom11121819.
A major barrier to the diagnosis and effective treatment of solid-tumor cancers is the difficulty in detection and visualization of tumor margins in primary and metastatic disease. The use of fluorescence can augment the surgeon's ability to detect cancer and aid in its resection. Several cancer types express carcinoembryonic antigen (CEA) including colorectal, pancreatic and gastric cancer. Antibodies to CEA have been developed and tagged with near-infrared fluorescent dyes. This review article surveyed the use of CEA antibodies conjugated to fluorescent probes for in vivo studies since 1990. PubMed and Google Scholar databases were queried, and 900 titles and abstracts were screened. Fifty-nine entries were identified as possibly meeting inclusion/exclusion criteria and were reviewed in full. Forty articles were included in the review and their citations were screened for additional entries. A total of 44 articles were included in the final review. The use of fluorescent anti-CEA antibodies has been shown to improve detection and resection of tumors in both murine models and clinically. The cumulative results indicate that fluorescent-conjugated anti-CEA antibodies have important potential to improve cancer diagnosis and surgery. In an emerging technology, anti-CEA fluorescent antibodies have also been successfully used for photoimmunotherapy treatment for cancer.
实体瘤癌症的诊断和有效治疗的主要障碍是在原发性和转移性疾病中难以检测和可视化肿瘤边界。荧光的使用可以增强外科医生检测癌症的能力并有助于切除癌症。几种癌症类型表达癌胚抗原（CEA），包括结直肠癌、胰腺癌和胃癌。已经开发出针对 CEA 的抗体，并与近红外荧光染料标记。本文综述了自 1990 年以来用于体内研究的与荧光探针偶联的 CEA 抗体的使用情况。查询了 PubMed 和 Google Scholar 数据库，并筛选了 900 个标题和摘要。确定了 59 个条目可能符合纳入/排除标准，并进行了全面审查。共有 40 篇文章被纳入综述，并对其引文进行了筛选以获取其他条目。共有 44 篇文章被纳入最终综述。荧光抗 CEA 抗体的使用已被证明可以提高在鼠模型和临床中的肿瘤检测和切除率。累积结果表明，荧光偶联的抗 CEA 抗体具有改善癌症诊断和手术的重要潜力。在一项新兴技术中，抗 CEA 荧光抗体也已成功用于癌症的光免疫治疗。
