Ścieżyńska Aneta, Sobiepanek Anna, Kowalska Patrycja D, Soszyńska Marta, Łuszczyński Krzysztof, Grzywa Tomasz M, Krześniak Natalia, Góźdź Agata, Włodarski Paweł K, Galus Ryszard, Kobiela Tomasz, Malejczyk Jacek
Department of Histology and Embryology, Medical University of Warsaw, 02-004 Warsaw, Poland.
Laboratory of Experimental Immunology, Military Institute of Hygiene and Epidemiology, 01-163 Warsaw, Poland.
Cancers (Basel). 2021 Dec 13;13(24):6244. doi: 10.3390/cancers13246244.
The development of an effective method of melanocyte isolation and culture is necessary for basic and clinical studies concerning skin diseases, including skin pigmentation disorders and melanoma. In this paper, we describe a novel, non-enzymatic and effective method of skin melanocyte and metastatic melanoma cell isolation and culture (along with the spontaneous spheroid creation) from skin or lymph node explants. The method is based on the selective harvesting of melanocytes and melanoma cells emigrating from the cultured explants. Thereby, isolated cells retain their natural phenotypical features, such as expression of tyrosinase and Melan-A as well as melanin production and are not contaminated by keratinocytes and fibroblasts. Such melanocyte and melanoma cell cultures may be very useful for medical and cosmetology studies, including studies of antitumor therapies.
开发一种有效的黑素细胞分离和培养方法对于包括皮肤色素沉着紊乱和黑色素瘤在内的皮肤病基础和临床研究至关重要。在本文中，我们描述了一种从皮肤或淋巴结外植体中分离和培养皮肤黑素细胞和转移性黑色素瘤细胞（以及自发形成球体）的新颖、非酶促且有效的方法。该方法基于从培养的外植体中选择性收获迁出的黑素细胞和黑色素瘤细胞。由此，分离出的细胞保留其自然表型特征，如酪氨酸酶和黑色素A的表达以及黑色素生成，且未被角质形成细胞和成纤维细胞污染。这种黑素细胞和黑色素瘤细胞培养物可能对医学和美容学研究非常有用，包括抗肿瘤治疗研究。
