Finney K J, Ince P, Appleton D R, Sunter J P, Watson A J
Cell Tissue Kinet. 1987 Jan;20(1):43-56. doi: 10.1111/j.1365-2184.1987.tb01081.x.
The development of an organ-culture system for rat colonic mucosa has enabled a direct assessment of the effect of epidermal growth factor (EGF) on cell division. An augmented mitotic index (AIm) has been employed to identify changes in cell proliferation. Explants of colonic mucosa from four animals were maintained in a medium containing serum for five days. On the fifth day of culture, half of the explants received fresh medium containing EGF (40 ng/ml) and the remainder (controls) fresh medium only. At 6, 12, 24 and 48 hr thereafter groups of both experimental and control explants received the metaphase-arresting drug vincristine (4 micrograms/ml) for 3 hr prior to fixation. The proportions of vincristine-arrested metaphases within the explants were determined. Analysis of the data indicates that when serum is present exogenous EGF exerts a trophic effect which increases with time (P less than 0.001). In a second experiment colonic explants from four animals were maintained for five days in a serum-free medium and were then divided into groups, each of which received one of a range of concentrations of EGF. The AIm was determined for each group after 36 hr. It was found that increasing concentrations of EGF produce a small but significant increase in cell proliferation (P less than 0.01). This effect, however, was less pronounced than that seen when serum was present. These results suggest that EGF has a trophic action on the colon and interacts with additional factors found in serum.
大鼠结肠黏膜器官培养系统的开发使得能够直接评估表皮生长因子（EGF）对细胞分裂的影响。已采用增强有丝分裂指数（AIm）来识别细胞增殖的变化。将来自四只动物的结肠黏膜外植体在含血清的培养基中培养五天。在培养的第五天，一半的外植体接受含有EGF（40 ng/ml）的新鲜培养基，其余（对照组）仅接受新鲜培养基。此后在6、12、24和48小时，实验组和对照组的外植体组在固定前3小时接受中期阻断药物长春新碱（4微克/毫升）。测定外植体内长春新碱阻断中期的比例。数据分析表明，当存在血清时，外源性EGF发挥营养作用，且该作用随时间增加（P小于0.001）。在第二个实验中，将来自四只动物的结肠外植体在无血清培养基中培养五天，然后分成几组，每组接受一系列浓度的EGF中的一种。36小时后测定每组的AIm。发现EGF浓度增加会使细胞增殖有小幅但显著的增加（P小于0.01）。然而，这种作用不如存在血清时明显。这些结果表明，EGF对结肠有营养作用，并与血清中发现的其他因子相互作用。
