McMannis J D, Goldman M B, Goldman J N
Cell Immunol. 1987 Apr 15;106(1):22-32. doi: 10.1016/0008-8749(87)90146-8.
Many laboratories have demonstrated that immunoglobulin production by B cells is controlled by networks of interacting lymphocytes and their products. Our laboratory has demonstrated that complement components produced by macrophages are also regulated by networks of interacting cells and humoral factors. Treatment of mice in vivo or guinea pig cells in vitro with anticomponent antibody specifically inhibits synthesis and secretion of the component by macrophages. We have further characterized the cellular basis for in vitro suppression of the fourth component of guinea pig complement. C4 suppression has been accomplished with dispersed spleen cells as well as intact splenic fragments. This facilitated examination of the cells responsible for long-term C4 suppression. The data suggested that C4 suppression required either cell contact or sufficient concentrations of soluble factors. Long-term suppression of C4 depends upon a lymphoid cell contained in the spleen and in lymph nodes but absent or in insufficient concentration in the peritoneum. The lymphocyte that actively maintains suppression was negative for the guinea pig T-cell marker detected by the monoclonal antibody mc8BE6. Therefore, the critical cell is either another T-cell subset or non-T lymphocyte. These data demonstrate that a network of interacting cells analogous to that proposed to regulate antibody synthesis is also involved in regulating some nonlymphoid cell products.
许多实验室已证明，B细胞产生免疫球蛋白是由相互作用的淋巴细胞及其产物网络所控制。我们实验室已证明，巨噬细胞产生的补体成分也受相互作用的细胞和体液因子网络的调节。用抗补体成分抗体在体内处理小鼠或在体外处理豚鼠细胞，可特异性抑制巨噬细胞对该成分的合成和分泌。我们进一步确定了体外抑制豚鼠补体第四成分的细胞基础。用分散的脾细胞以及完整的脾碎片均可实现对C4的抑制。这便于检查负责长期抑制C4的细胞。数据表明，抑制C4需要细胞接触或足够浓度的可溶性因子。对C4的长期抑制取决于脾脏和淋巴结中含有的一种淋巴细胞，但在腹膜中不存在或浓度不足。通过单克隆抗体mc8BE6检测，积极维持抑制作用的淋巴细胞对豚鼠T细胞标志物呈阴性。因此，关键细胞要么是另一个T细胞亚群，要么是非T淋巴细胞。这些数据表明，类似于调节抗体合成所提出的相互作用细胞网络，也参与调节某些非淋巴细胞产物。
