Gorczynski R M, Gregoris M, Boulanger M
Cell Immunol. 1987 Apr 15;106(1):88-99. doi: 10.1016/0008-8749(87)90152-3.
Limiting dilution cultures of alloreactive (anti-H2Kb) CTL were established from thymocyte or spleen cell pools of C3H/HeJ and their congenic bg/bg partner, or of SJL/J and their congenic bg/bg partner. CTL populations in these cultures were assayed for cross-reactive lysis of a panel of splenic Con A blasts of H2Kbm mutant mice. There was some slight elevation of frequency of CTLp in the thymocyte lymphoid pool of bg/bg mice; more strikingly, the repertoire of anti-H2Kb specificities was clearly altered in both strains in the bg/bg animals. There was apparently an increased diversity (more specificities represented at higher frequencies) in the thymocyte pool and a decreased diversity in the spleen cell CTLp pool in animals with the bg/bg marker. Similar shifts in the allorecognition repertoire of normal C3H/HeJ mice were produced by inoculation of neonatal mice with a rabbit anti-NK heteroantibody (antiasialo GM1). Preabsorption of this serum such that it lost anti-NK activity also abolished this effect of in vivo neonatal injection. Furthermore, injection of bg/bg bone marrow-reconstituted C3H/HeJ (bg/+) mice with a C3H spleen cell-derived NK line also caused a shift in the allorecognition repertoire toward that seen in the normal littermate control animals.
从C3H/HeJ及其同基因bg/bg伙伴，或SJL/J及其同基因bg/bg伙伴的胸腺细胞或脾细胞库中建立同种反应性（抗H2Kb）CTL的有限稀释培养物。检测这些培养物中的CTL群体对一组H2Kbm突变小鼠脾Con A母细胞的交叉反应性裂解。bg/bg小鼠胸腺细胞淋巴库中CTLp频率略有升高；更显著的是，在bg/bg动物的两个品系中，抗H2Kb特异性的库明显改变。在具有bg/bg标记的动物中，胸腺细胞库中显然多样性增加（更多特异性以更高频率出现），而脾细胞CTLp库中多样性降低。用兔抗NK异种抗体（抗唾液酸GM1）接种新生小鼠可使正常C3H/HeJ小鼠的同种识别库产生类似变化。预先吸收该血清使其失去抗NK活性也消除了体内新生注射的这种效应。此外，用C3H脾细胞来源的NK系注射bg/bg骨髓重建的C3H/HeJ（bg/+）小鼠也导致同种识别库向正常同窝对照动物所见的方向转变。
