Hwang Hee-Jeong, Kim Yu-Gyeong, Chung Myong-Soo
Research Institute of Biotechnology and Medical Converged Science, Dongguk University, Seoul 10326, Korea.
Department of Food Science and Engineering, Ewha Womans University, Seoul 03760, Korea.
Foods. 2021 Dec 13;10(12):3092. doi: 10.3390/foods10123092.
The aim of this study was to find the optimum condition of pulsed electric field (PEF) and intense pulsed light (IPL) for the enhancement of subcritical water extraction (SWE), which is an eco-friendly extraction method, for extracting tea catechins from green tea leaves (). The leaves were treated with PEF under conditions of electric field strength (1, 2 and 3 kV/cm) during 60 s. Moreover, IPL was applied at various voltages (800, 1000, and 1200 V) for 60 s. The SWE was performed for 5 min at varying temperatures (110, 130, 150, 170, and 190 °C). The maximum yield of total catechin was 44.35 ± 2.00 mg/g dry green tea leaves at PEF treatment conditions of 2 kV/cm during 60 s, as well as the SWE temperature of 130 °C. In the case of IPL treatment, the largest amount of total catechin was 48.06 ± 5.03 mg/g dry green tea leaves at 800 V during 60 s when the extraction temperature was 130 °C. The total catechin content was increased by 15.43% for PEF and 25.09% for IPL compared to the value of untreated leaves. This study verified that PEF and IPL had a positive effect on the enhancement of tea catechins extraction from green tea leaves using SWE.
本研究的目的是找出脉冲电场(PEF)和强脉冲光(IPL)用于强化亚临界水萃取(SWE,一种环保型萃取方法)从绿茶叶片中提取茶儿茶素的最佳条件。叶片在电场强度为1、2和3 kV/cm的条件下接受PEF处理60秒。此外,IPL在不同电压(800、1000和1200 V)下施加60秒。SWE在不同温度(110、130、150、170和190℃)下进行5分钟。在PEF处理条件为2 kV/cm持续60秒以及SWE温度为130℃时,总儿茶素的最大产率为44.35±2.00 mg/g干绿茶叶片。在IPL处理的情况下,当萃取温度为130℃时,在800 V持续60秒的条件下,总儿茶素的最大量为48.06±5.03 mg/g干绿茶叶片。与未处理叶片的值相比,PEF使总儿茶素含量提高了15.43%,IPL使总儿茶素含量提高了25.09%。本研究证实,PEF和IPL对使用SWE从绿茶叶片中强化提取茶儿茶素有积极作用。