Boyd W H, Jichici D, Sathasivam P
Endocrinol Exp. 1987 Mar;21(1):31-42.
The purpose of this study was to determine the presence of immunoglobulin G in bovine pituitary intraglandular colloid (IGC). Intraglandular colloid, housed in the intraglandular lumen (residual lumen), is the holocrine secretion of the marginal layers of intermediate lobe (IL) cells. Immunoglobulin G was detected and quantitated in bovine IGC using an Enzyme Linked Immunosorbent Assay (ELISA). This study establishes the presence of immunoglobulin G in the intraglandular colloid at levels that are of the order of 10 micrograms per mg of colloid. The existence of this molecule in material scrupulously prepared to exclude the possibility of serum contamination in this normally avascular compartment is a unique phenomenon.
本研究的目的是确定牛垂体腺内胶体(IGC)中免疫球蛋白G的存在情况。腺内胶体存在于腺内腔(残留腔)中,是中间叶(IL)细胞边缘层的全分泌产物。使用酶联免疫吸附测定(ELISA)对牛IGC中的免疫球蛋白G进行检测和定量。本研究证实,腺内胶体中存在免疫球蛋白G,其含量约为每毫克胶体10微克。在这个通常无血管的腔室中,经过严格制备以排除血清污染可能性的物质中存在这种分子,这是一个独特的现象。