Akeson A L, Harmony J A
Exp Cell Res. 1987 May;170(1):129-46. doi: 10.1016/0014-4827(87)90122-4.
The response of highly enriched populations of human T8+ lymphocytes to the oxidative mitogenic enzymes neuraminidase (NA) and galactose oxidase (GO) was enhanced by NAGO-primed T4+ lymphocytes. No similar enhancement occurred when the cells were primed with phytohemagglutinin (PHA). In the absence of subclass contamination (1%), the T8+ and T4+ cells responded equally to NAGO by the criterion of DNA replication. The addition of a small number, 2-10%, of NAGO-T4+ cells to the NAGO-T8+ cells enhanced DNA synthesis by as much as 8.5-fold. Augmentation of the cellular response did not occur unless the T4+ cells were activated by NAGO. The converse situation, 2-10% of NAGO-T8+ cells in a primarily NAGO-T4+ cell population, did not increase the DNA synthetic response of the NAGO-T4+ cells. The NAGO-T4+ cells did not augment the early event of increased phosphatidylinositol metabolism or the midcycle event of induction of receptors for interleukin 2 (IL2) and transferrin. The NAGO-T4+ cells therefore increased the probability that fully activated T8+ lymphocytes crossed the G1/S boundary. The basis for this effect was not an enhanced responsiveness of the NAGO-T8+ cells to IL2 or to other soluble growth mediators in medium conditioned by NAGO-activated lymphocytes. The results of this investigation thus implicate a control point in the NAGO-T8+ lymphocyte cell cycle that is positively modulated by the NAGO-T4+ cells themselves or by a product of their activation.
经NAGO预刺激的T4 +淋巴细胞增强了高度富集的人T8 +淋巴细胞群体对氧化促有丝分裂酶神经氨酸酶（NA）和半乳糖氧化酶（GO）的反应。当细胞用植物血凝素（PHA）预刺激时，未发生类似的增强作用。在不存在亚类污染（1%）的情况下，根据DNA复制标准，T8 +和T4 +细胞对NAGO的反应相同。向NAGO - T8 +细胞中添加少量（2 - 10%）的NAGO - T4 +细胞可使DNA合成增强多达8.5倍。除非T4 +细胞被NAGO激活，否则不会发生细胞反应的增强。相反的情况，即在主要为NAGO - T4 +细胞群体中加入2 - 10%的NAGO - T8 +细胞，并未增加NAGO - T4 +细胞的DNA合成反应。NAGO - T4 +细胞并未增强磷脂酰肌醇代谢增加的早期事件或白细胞介素2（IL2）和转铁蛋白受体诱导的中期事件。因此，NAGO - T4 +细胞增加了完全活化的T8 +淋巴细胞跨越G1 / S边界的概率。这种效应的基础不是NAGO - T8 +细胞对IL2或对由NAGO激活的淋巴细胞条件培养基中的其他可溶性生长介质的反应性增强。因此，本研究结果表明在NAGO - T8 +淋巴细胞细胞周期中存在一个控制点，该控制点受到NAGO - T4 +细胞本身或其活化产物的正向调节。
