Novogrodsky A, Suthanthiran M, Saltz B, Newman D, Rubin A L, Stenzel K H
J Exp Med. 1980 Mar 1;151(3):755-60. doi: 10.1084/jem.151.3.755.
Supernates of neuraminidase and galactose oxidase (NAGO)-treated lymphocytes induce blastogenesis in nonproliferating cells harvested 7--14 d after treatment with mitogen or alloantigen and in cells incubated with mitogen for 7--14 d but not in freshly isolated peripheral blood lymphocytes9 Virtually all the growth factor is produced by NAGO-treated cells during the first 24 h of incubation, and no increase in factor activity is detected upon further cell culture. Serum is not required for growth factor production. NAGO-primed medium induces generation of specific cytotoxic T cells from mixed lymphocyte culture (MLC) memory cells to approximately the same extent as that induced by allogeneic cells (stimulating cells in the primary MLC). NAGO-primed medium provides a useful reagent for isolation and characterization of lymphocyte growth factors and other lymphokines.
经神经氨酸酶和半乳糖氧化酶(NAGO)处理的淋巴细胞的上清液,可诱导在经丝裂原或同种异体抗原处理7 - 14天后收获的非增殖细胞以及与丝裂原孵育7 - 14天的细胞发生母细胞化,但对新鲜分离的外周血淋巴细胞无此作用。实际上,所有生长因子都是在孵育的最初24小时内由NAGO处理的细胞产生的,进一步细胞培养后未检测到因子活性增加。生长因子的产生不需要血清。NAGO预处理的培养基诱导混合淋巴细胞培养(MLC)记忆细胞产生特异性细胞毒性T细胞的程度,与同种异体细胞(初次MLC中的刺激细胞)诱导的程度大致相同。NAGO预处理的培养基为淋巴细胞生长因子和其他淋巴因子的分离和表征提供了一种有用的试剂。