淋巴因子激活的杀伤细胞的产生不需要DNA合成。
Generation of lymphokine-activated killer cells does not require DNA synthesis.
作者信息
Malkovský M, Jíra M, Madar J, Malkovska V, Loveland B, Asherson G L
出版信息
Immunology. 1987 Mar;60(3):471-3.
Abstract
We studied the role of DNA synthesis in the induction of lymphokine-activated killer (LAK) cells by recombinant interleukin-2 (IL-2) and the dependence of this phenomenon on DNA synthesis. Doses of gamma-irradiation (1000-5000 rads) that profoundly reduced DNA synthesis in human peripheral blood mononuclear leucocytes (PBL) also effectively suppressed the development of cytotoxic activity in the absence of IL-2. However, the same doses of irradiation affected the induction of LAK activity by IL-2 to a much lesser extent. Blocking the formation of deoxyribonucleotides by hydroxyurea, which resulted in a complete inhibition of DNA synthesis in PBL or purified T lymphocytes, had virtually no effect on the generation of LAK cells. These results indicate that the expression of LAK activity is not dependent on DNA synthesis.
摘要
我们研究了DNA合成在重组白细胞介素-2（IL-2）诱导淋巴因子激活的杀伤（LAK）细胞中的作用以及这一现象对DNA合成的依赖性。能显著降低人外周血单个核白细胞（PBL）中DNA合成的γ射线剂量（1000 - 5000拉德），在无IL-2的情况下也能有效抑制细胞毒性活性的发展。然而，相同剂量的辐射对IL-2诱导LAK活性的影响要小得多。羟基脲阻断脱氧核糖核苷酸的形成，这导致PBL或纯化的T淋巴细胞中DNA合成完全受到抑制，但对LAK细胞的产生几乎没有影响。这些结果表明，LAK活性的表达不依赖于DNA合成。
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