Bobé P, Gachelin G, Kiger N
Immunogenetics. 1987;25(4):251-7. doi: 10.1007/BF00404695.
The MRL/lpr (H-2k) inbred strain, a model for the autoimmune disease systemic lupus erythematosus, differs from the healthy inbred strain MRL +/+ (H-2k) by only 0.1% of its genome. Southern blot analysis using class I and class II probes confirmed the H-2k genotype of both strains. Among the Iak-positive peritoneal cells, cells with an unexpected expression of Iad specificities were detected in a radioimmunoassay using several monoclonal antibodies and one conventional antiserum. This was only found in aged (6- to 9-month-old) mice both in the MRL/lpr strain (32% Iad-positive mice) and in the MRL +/+ strain (42% Iad-positive mice). Furthermore, 24% of aged MRL/lpr mice exhibited strong spontaneous cytotoxic T lymphocyte (CTL) activities against P815 (H-2d) target cells, and 57% had a weaker but still detectable level of cytotoxicity. In contrast, such a CTL activity has never been found in the MRL +/+ strain. These results suggest that the anti-H-2d CTL plays a role in the onset of the autoimmune process in MRL/lpr mice.
MRL/lpr（H-2k）近交系是自身免疫性疾病系统性红斑狼疮的一种模型，它与健康近交系MRL +/+（H-2k）仅在0.1%的基因组上存在差异。使用I类和II类探针的Southern印迹分析证实了两个品系的H-2k基因型。在Iak阳性的腹膜细胞中，使用几种单克隆抗体和一种传统抗血清进行放射免疫测定时，检测到了具有意外Iad特异性表达的细胞。这种情况仅在老年（6至9个月大）小鼠中发现，在MRL/lpr品系（32%的Iad阳性小鼠）和MRL +/+品系（42%的Iad阳性小鼠）中均有出现。此外，24%的老年MRL/lpr小鼠对P815（H-2d）靶细胞表现出强烈的自发细胞毒性T淋巴细胞（CTL）活性，57%的小鼠具有较弱但仍可检测到的细胞毒性水平。相比之下，在MRL +/+品系中从未发现过这种CTL活性。这些结果表明，抗H-2d CTL在MRL/lpr小鼠自身免疫过程的发病中起作用。
