Chen Yanan, Cao Yingzi, Gai Yunpeng, Ma Haijie, Zhu Zengrong, Chung Kuang-Ren, Li Hongye
The Key Laboratory of Molecular Biology of Crop Pathogens and Insects of Ministry of Agriculture and Rural Affairs, The Key Laboratory of Biology of Crop Pathogens and Insects of Zhejiang Province, Institute of Biotechnology, Zhejiang University, Hangzhou 310058, China.
School of Agriculture and Food Sciences, Zhejiang Agriculture & Forestry University, Hangzhou 311300, China.
J Fungi (Basel). 2021 Nov 26;7(12):1013. doi: 10.3390/jof7121013.
In the present study, we identified six GATA transcription factors (AaAreA, AaAreB, AaLreA, AaLreB, AaNsdD, and AaSreA) and characterized their functions in response to environmental stress and virulence in the tangerine pathotype of . The targeted gene knockout of each of the GATA-coding genes decreased the growth to varying degrees. The mutation of , , , or decreased the conidiation. All the GATA transcription factors were found to be required for tolerance to cumyl hydroperoxide and tert-butyl-hydroperoxide (oxidants) and Congo red (a cell-wall-destructing agent). Pathogenicity assays assessed on detached citrus leaves revealed that mutations of , , , or significantly decreased the fungal virulence. A comparative transcriptome analysis between the ∆ mutant and the wild-type strain revealed that the inactivation of led to alterations in the expression of genes involved in a number of biological processes, including oxidoreductase activity, amino acid metabolism, and secondary metabolite biogenesis. Taken together, our findings revealed that GATA-coding genes play diverse roles in response to environmental stress and are important regulators involved in fungal development, conidiation, ROS detoxification, as well as pathogenesis. This study, for the first time, systemically underlines the critical role of GATA transcription factors in response to environmental stress and virulence in .
在本研究中，我们鉴定出六个GATA转录因子（AaAreA、AaAreB、AaLreA、AaLreB、AaNsdD和AaSreA），并对它们在柑橘溃疡病菌致病型中响应环境胁迫和毒力方面的功能进行了表征。每个编码GATA的基因的靶向基因敲除均不同程度地降低了生长。AaAreA、AaAreB、AaLreA或AaLreB的突变降低了分生孢子形成。发现所有GATA转录因子对于耐受氢过氧化异丙苯和叔丁基过氧化氢（氧化剂）以及刚果红（一种细胞壁破坏剂）都是必需的。在离体柑橘叶片上进行的致病性测定表明，AaAreA、AaAreB、AaLreA或AaLreB的突变显著降低了真菌毒力。∆突变体与野生型菌株之间的比较转录组分析表明，AaAreA的失活导致参与许多生物学过程的基因表达发生改变，包括氧化还原酶活性、氨基酸代谢和次生代谢物生物合成。综上所述，我们的研究结果表明，编码GATA的基因在响应环境胁迫中发挥多种作用，并且是参与真菌发育、分生孢子形成、活性氧解毒以及致病过程的重要调节因子。本研究首次系统地强调了GATA转录因子在柑橘溃疡病菌响应环境胁迫和毒力方面的关键作用。
