Tian Ye, Wei Ming, Wang Lijun, Hong Yuankai, Luo Dan, Sha Yinlin
Department of Biophysics, Single-Molecule and Nanobiology Laboratory, School of Basic Medical Sciences, Peking University, Beijing 100191, China.
CAS Center for Excellence in Nanoscience, Beijing Key Laboratory of Micro-Nano Energy and Sensor, Beijing Institute of Nanoenergy and Nanosystems, Chinese Academy of Sciences, Beijing 100083, China.
Materials (Basel). 2021 Dec 15;14(24):7744. doi: 10.3390/ma14247744.
Due to the unique advantages of two-photon technology and time-resolved imaging technology in the biomedical field, attention has been paid to them. Gold clusters possess excellent physicochemical properties and low biotoxicity, which make them greatly advantageous in biological imaging, especially for in vivo animal imaging. A gold nanocluster was coupled with dihydrolipoic acid to obtain a functionalized nanoprobe; the material displayed significant features, including a large two-photon absorption cross-section (up to 1.59 × 10 GM) and prolonged fluorescence lifetime (>300 ns). The two-photon and time-resolution techniques were used to perform cell imaging and in vivo imaging.
由于双光子技术和时间分辨成像技术在生物医学领域具有独特优势，它们受到了关注。金簇具有优异的物理化学性质和低生物毒性，这使得它们在生物成像尤其是体内动物成像方面具有很大优势。将金纳米簇与二氢硫辛酸偶联，得到功能化纳米探针；该材料具有显著特性，包括大的双光子吸收截面（高达1.59×10 GM）和延长的荧光寿命（>300 ns）。利用双光子和时间分辨技术进行细胞成像和体内成像。
