Xu Zhencai, Song Aibo, Wang Fangwu, Chen Hongwei
Guanyun People's Hospital Lianyungang 222000 China
Department of Otolaryngology, Head and Neck Surgery, The First Affiliated Hospital of Hainan Medical University Haikou 570102 China
RSC Adv. 2021 Sep 30;11(51):32203-32209. doi: 10.1039/d1ra06052j. eCollection 2021 Sep 27.
CO, a gas molecule that is harmful to living organisms, has a high affinity with hemoglobin, which will cause severe hypoxia. However, in recent years, researchers have discovered that endogenous CO, similar to NO, is one of the messenger molecules, which has a certain regulatory effect in many physiological and pathological processes in the respiratory system, cardiovascular system, and nervous system. Therefore, it is urgent to explore an effective method to monitor the role of CO under physiological and pathological conditions. Herein, we designed and synthesized a near-infrared small-molecule fluorescent probe for the detection of CO in living cells. In this design, a two-site BODIPY dye was introduced as the fluorophore, and the allyl chloroformate part as the CO reactive group. The probe displays excellent sensitivity, selectivity, and a good linear relationship to CO. Furthermore, it shows good biocompatibility and low cytotoxicity. This probe has been successfully applied to the detection of CO in a variety of cells. The developed fluorescent probe can serve as a potential molecular imaging tool for imaging and detection of CO.
一氧化碳是一种对生物有害的气体分子，它与血红蛋白具有很高的亲和力，会导致严重缺氧。然而，近年来研究人员发现，内源性一氧化碳与一氧化氮类似，是信使分子之一，在呼吸系统、心血管系统和神经系统的许多生理和病理过程中具有一定的调节作用。因此，迫切需要探索一种有效的方法来监测一氧化碳在生理和病理条件下的作用。在此，我们设计并合成了一种用于检测活细胞中一氧化碳的近红外小分子荧光探针。在该设计中，引入了一种双位点硼二吡咯染料作为荧光团，并将氯甲酸烯丙酯部分作为与一氧化碳反应的基团。该探针表现出优异的灵敏度、选择性，并且与一氧化碳具有良好的线性关系。此外，它还显示出良好的生物相容性和低细胞毒性。该探针已成功应用于多种细胞中一氧化碳的检测。所开发的荧光探针可作为一种潜在的分子成像工具用于一氧化碳的成像和检测。
