Alley C D
J Clin Immunol. 1987 Mar;7(2):151-8. doi: 10.1007/BF00916009.
Although human bone marrow has been implicated in the production of serum immunoglobulins, little information is available concerning the kinetics of antibody production (primary- or secondary-type humoral response) or the cells that are responsible for antibody production in human bone marrow. In this study, the kinetics of and cells responsible for antibody production in the bone marrow were investigated. Previous studies have demonstrated that human bone marrow mononuclear cells secrete a significant amount of IgA in vitro. This finding led to the focus of the present investigation on bone marrow IgA production. The results reported here demonstrate that IgA was synthesized de novo in cultures of bone marrow mononuclear cells; its peak concentration in the culture supernatants preceded that of IgM; its production was totally inhibited by the addition of microgram quantities of anti-alpha-chain antiserum, while milligram quantities of anti-mu-chain antiserum were required for even partial inhibition of IgA production; and the culture of isolated IgA-bearing cells resulted in a greater than 13-fold increase in IgA concentration in the culture supernatants as compared with those from unseparated bone marrow mononuclear cells. From this study, it was concluded that bone marrow produces IgA as a secondary or anamnestic response to some undetermined stimulus and that IgA-committed B cells residing in, although probably not stimulated in, the bone marrow compartment were responsible for the IgA synthesis and secretion in vitro.
尽管已有研究表明人类骨髓参与血清免疫球蛋白的产生，但关于抗体产生的动力学（初次或二次体液免疫反应）以及人类骨髓中负责抗体产生的细胞，目前所知甚少。在本研究中，我们对骨髓中抗体产生的动力学以及负责抗体产生的细胞进行了研究。此前的研究已证明，人类骨髓单个核细胞在体外可分泌大量IgA。这一发现使得本研究聚焦于骨髓IgA的产生。此处报告的结果表明，IgA在骨髓单个核细胞培养物中是重新合成的；其在培养上清液中的峰值浓度先于IgM出现；添加微克量的抗α链抗血清可完全抑制其产生，而即使是部分抑制IgA的产生也需要毫克量的抗μ链抗血清；与未分离的骨髓单个核细胞相比，分离出的携带IgA的细胞培养物可使培养上清液中的IgA浓度增加超过13倍。从本研究得出的结论是，骨髓作为对某种未确定刺激的二次或回忆性反应产生IgA，并且存在于骨髓区室中的IgA特异性B细胞（尽管可能未受到刺激）负责体外IgA的合成和分泌。
