Corbel C, Belo M, Martin C, Le Douarin N M
J Immunol. 1987 May 1;138(9):2813-21.
Two methods to bursectomize chick embryos before hemopoietic cell seeding of the bursa of Fabricius were compared in this work: section of the tail region at E3 including the presumptive bursal territory, and selective removal of the bursa at E5. Hatching ability is better with the former method, but survival rate and effectiveness of bursectomy are favored with the second, novel technique. Moreover, selective removal of the bursa at E5 can be followed by in situ engraftment of a quail bursa and construction of quail-chick bursal chimeras. The immune response of bursaless birds and bursal chimeras has been studied. Total absence of the bursa does not prevent a few B cells from differentiating and nonspecific Ig (IgM and/or IgG) from being secreted. As reported previously, bursaless birds, however, are unable to mount an immune response by producing specific antibodies. This immune function is restored by the graft of a quail bursa. The microenvironment of the bursa, although heterospecific, allows the expansion of the B cell population and generates the repertoire of the B cell antigen receptors. This process takes place during late embryonic and early postnatal life because the grafted quail bursal stroma is subjected to immune rejection from 2 to 3 wk after birth in all chimeras, which are, however, perfectly immunocompetent.
在这项研究中，我们比较了两种在法氏囊造血细胞接种前切除雏鸡胚胎法氏囊的方法：在胚胎第3天（E3）切除包括假定法氏囊区域的尾部区域，以及在胚胎第5天（E5）选择性切除法氏囊。前一种方法孵化能力更好，但后一种新技术在法氏囊切除的存活率和有效性方面更具优势。此外，在E5选择性切除法氏囊后，可以原位植入鹌鹑法氏囊并构建鹌鹑 - 鸡法氏囊嵌合体。我们对无囊鸟类和囊嵌合体的免疫反应进行了研究。法氏囊完全缺失并不妨碍少数B细胞分化以及分泌非特异性免疫球蛋白（IgM和/或IgG）。然而，如先前报道的那样，无囊鸟类无法通过产生特异性抗体来引发免疫反应。通过移植鹌鹑法氏囊可恢复这种免疫功能。法氏囊的微环境虽然是异种特异性的，但能使B细胞群体扩增并产生B细胞抗原受体库。这个过程发生在胚胎后期和出生后早期，因为移植的鹌鹑法氏囊基质在所有嵌合体出生后2至3周会受到免疫排斥，不过这些嵌合体具有完全的免疫能力。
