Belo M, Corbel C, Martin C, Le Douarin N M
Institut d'Embryologie cellulaire et moleculaire du CNRS, Nogent-sur-Marne, France.
Int Immunol. 1989;1(2):105-12. doi: 10.1093/intimm/1.2.105.
We demonstrated previously that isotopic and isochronic grafts of the quail bursa of Fabricius rudiment performed at 5 days of incubation (E5) into chick embryos resulted in the development of a chimeric bursa whose chick host B lymphocytes and accessory cells differentiated in a foreign, quail epithelial environment. Such animals reject their grafted bursa by the age of 2-3 weeks post-hatching (1,2). Isotopic embryonic grafts of the thymus epitheliomesenchymal anlagen from the quail donor of the bursal rudiment were carried out at E4.5 (before their colonization by hemopoietic precursor cells), following partial or complete host thymectomy. The quail thymic epithelial stroma was accepted and invaded by chick hemopoietic precursor cells that further differentiated into lymphocytes and dendritic cells. Tolerance of the foreign bursa was induced in such thymobursal chimeras. This demonstrates that the thymic epithelium has the capacity to induce tolerance of xenogeneic rudiments when both grafts are implanted at early stages of embryonic development. We also report on the production of two birds in which removal of the chick host thymus was complete thus generating chimeras in which host T and B lymphocytes differentiated in a completely xenogeneic epithelial environment.
我们之前证明，在孵化第5天（E5）将鹌鹑法氏囊原基进行同位素和等时移植到鸡胚中，会导致嵌合法氏囊的发育，其鸡宿主B淋巴细胞和辅助细胞在异源的鹌鹑上皮环境中分化。此类动物在孵化后2至3周龄时会排斥移植的法氏囊（1,2）。在部分或完全切除宿主胸腺后，于E4.5（在造血前体细胞定植之前）进行来自法氏囊原基鹌鹑供体的胸腺上皮间充质原基的同位素胚胎移植。鹌鹑胸腺上皮基质被鸡造血前体细胞接受并侵入，这些细胞进一步分化为淋巴细胞和树突状细胞。在这种胸腺 - 法氏囊嵌合体中诱导了对外源法氏囊的耐受性。这表明，当两种移植都在胚胎发育早期植入时，胸腺上皮具有诱导对异种原基耐受性的能力。我们还报告了两只鸡的产生情况，其中鸡宿主胸腺的切除是完全的，从而产生了嵌合体，其中宿主T和B淋巴细胞在完全异源的上皮环境中分化。
