Department of Otolaryngology-Head and Neck Surgery, University of Miami Leonard Miller School of Medicine, Miami, FL 33136, USA.
Herbert Wertheim College of Medicine, Florida International University, Miami, FL 33199, USA.
Int J Environ Res Public Health. 2021 Dec 14;18(24):13174. doi: 10.3390/ijerph182413174.
Tobacco is a risk factor of head and neck cancer (HNC) and smoking cessation alone may reduce HNC risk by 70%. Soluble CD44 (solCD44), a cell surface receptor linked to cell proliferation and migration, and total protein (TP) levels can detect early HNC. This study aims to determine whether salivary solCD44 and TP levels in oral rinses change following a smoking cessation program. 150 smokers provided oral rinse samples at baseline and at a 12-month follow-up after participation in a smoking cessation program. Assays to measure levels of solCD44, TP, and cotinine, a metabolite used as a biomarker of tobacco exposure, were completed. A paired-samples t-test was used to determine whether there was a statistically significant ( < 0.05) mean difference in biomarker levels before and after the program. Baseline and at 12-month follow-up data were available for 88 subjects, 21 of whom quit smoking entirely. Mean levels of solCD44 significantly decreased by 0.412 ng/mL from baseline to the 12-month follow-up, = 0.010. There was no significant difference in mean TP levels, = 0.975. Mean cotinine levels decreased significantly by 74.7 ng/mL, = 0.035. This is the first work demonstrating an association between smoking cessation and decreased solCD44 levels in oral rinses. Decreased expression of the tumorigenic CD44 may be one mechanism by which smoking cessation lowers cancer risk.
烟草是头颈部癌症（HNC）的一个风险因素，单独戒烟可使 HNC 风险降低 70%。可溶性 CD44（solCD44）是一种与细胞增殖和迁移相关的细胞表面受体，总蛋白（TP）水平可检测早期 HNC。本研究旨在确定戒烟计划后漱口液中的唾液可溶性 CD44 和 TP 水平是否发生变化。150 名吸烟者在参与戒烟计划前和 12 个月随访时提供了漱口样本。完成了测量 solCD44、TP 和可替宁（一种用于检测烟草暴露的生物标志物）水平的测定。使用配对样本 t 检验确定在计划前后生物标志物水平是否存在统计学上显著差异（<0.05）。88 名受试者中有 21 名完全戒烟，他们的基线和 12 个月随访数据可用。与基线相比，12 个月随访时 solCD44 的平均水平显著下降了 0.412ng/mL，=0.010。TP 平均水平无显著差异，=0.975。可替宁平均水平显著下降了 74.7ng/mL，=0.035。这是首次证明戒烟与漱口液中 solCD44 水平降低之间存在关联的工作。肿瘤发生 CD44 表达的减少可能是戒烟降低癌症风险的一种机制。
