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通过低能耗的解磷途径工程化发酵纤维二糖对同步糖化发酵的影响。

Effects of Engineered Fermenting Cellobiose through Low-Energy-Consuming Phosphorolytic Pathway in Simultaneous Saccharification and Fermentation.

机构信息

Department of Bioenergy Science and Technology, and Department of Integrative Food, Bioscience and Biotechnology, Chonnam National University, Gwangju 61186, Republic of Korea.

Department of Food Science and Human Nutrition, and Carl R. Woese Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA.

出版信息

J Microbiol Biotechnol. 2022 Jan 28;32(1):117-125. doi: 10.4014/jmb.2111.11047.

DOI:10.4014/jmb.2111.11047
PMID:34949751
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9628822/
Abstract

Until recently, four types of cellobiose-fermenting strains have been developed by introduction of a cellobiose metabolic pathway based on either intracellular β-glucosidase (GH1-1) or cellobiose phosphorylase (CBP), along with either an energy-consuming active cellodextrin transporter (CDT-1) or a non-energy-consuming passive cellodextrin facilitator (CDT-2). In this study, the ethanol production performance of two cellobiose-fermenting strains expressing mutant CDT-2 (N306I) with GH1-1 or CBP were compared with two cellobiose-fermenting strains expressing mutant CDT-1 (F213L) with GH1-1 or CBP in the simultaneous saccharification and fermentation (SSF) of cellulose under various conditions. It was found that, regardless of the SSF conditions, the phosphorolytic cellobiose-fermenting expressing mutant CDT-2 with CBP showed the best ethanol production among the four strains. In addition, during SSF contaminated by lactic acid bacteria, the phosphorolytic cellobiose-fermenting expressing mutant CDT-2 with CBP showed the highest ethanol production and the lowest lactate formation compared with those of other strains, such as the hydrolytic cellobiose-fermenting expressing mutant CDT-1 with GH1-1, and the glucose-fermenting with extracellular β-glucosidase. These results suggest that the cellobiose-fermenting yeast strain exhibiting low energy consumption can enhance the efficiency of the SSF of cellulosic biomass.

摘要

直到最近，通过引入基于细胞内β-葡萄糖苷酶 (GH1-1) 或纤维二糖磷酸化酶 (CBP) 的细胞二糖代谢途径，以及能量消耗型主动纤维二糖转运蛋白 (CDT-1) 或非能量消耗型被动纤维二糖促进剂 (CDT-2)，已经开发出了四种能够发酵纤维二糖的菌株。在这项研究中，比较了两种表达突变型 CDT-2 (N306I) 的纤维二糖发酵菌株（一种与 GH1-1 或 CBP 共表达，另一种与 GH1-1 或 CBP 共表达）与两种表达突变型 CDT-1 (F213L) 的纤维二糖发酵菌株在不同条件下进行纤维素同步糖化发酵 (SSF) 的乙醇生产性能。结果发现，无论 SSF 条件如何，具有 CBP 的磷酸解纤维二糖发酵菌株表达突变型 CDT-2 均表现出最佳的乙醇生产性能。此外，在受乳酸菌污染的 SSF 中，与其他菌株（如具有 GH1-1 的水解纤维二糖发酵菌株和具有细胞外 β-葡萄糖苷酶的葡萄糖发酵菌株）相比，具有 CBP 的磷酸解纤维二糖发酵菌株表达突变型 CDT-2 表现出最高的乙醇产量和最低的乳酸盐形成。这些结果表明，具有低能量消耗的纤维二糖发酵酵母菌株可以提高纤维素生物质 SSF 的效率。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b2c1/9628822/ceb2be576b56/jmb-32-1-117-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b2c1/9628822/3d9cf5851451/jmb-32-1-117-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b2c1/9628822/f9934f167064/jmb-32-1-117-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b2c1/9628822/ceb2be576b56/jmb-32-1-117-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b2c1/9628822/3d9cf5851451/jmb-32-1-117-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b2c1/9628822/f9934f167064/jmb-32-1-117-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b2c1/9628822/ceb2be576b56/jmb-32-1-117-f3.jpg

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