Wahren B, Hammarström S, Harmenberg U, von Krusenstierna S, Larsson S A, Lundell G, Rudén U, Schnell P O
Tumour Biol. 1986;7(5-6):361-9.
Antibodies to carcinoembryonic antigen (CEA) from sheep and monkey were immunoadsorbent purified. Mouse monoclonal antibody (MAb) anti-CEA I-38S1 and Fab fragments of this antibody were prepared from mouse ascitic fluid. The IgG preparations were labelled with 123I or 131I, the Fab fragments with 131I. The antibody reactivity was unchanged after labelling. Patients with advanced colorectal carcinomas received an intravenous injection of 50-200 MBq 123I or 30-160 MBq 131I coupled to 250-500 micrograms antibody or antibody fragment. Patient examinations were performed using emission tomography (SPECT) and/or conventional gamma camera scintigraphy. The specific localization of labelled anti-CEA to tumor was compared to known tumor localized by CAT-scan, other x-ray methods or laparotomy, 50% of known tumors were accurately localized with sheep anti-CEA. In contrast, 70-80% of known tumor sites were correctly localized with polyclonal monkey anti-CEA antibodies, with monoclonal anti-CEA antibodies or with Fab fragments of the latter. A few previously unknown tumors were detected.
对来自绵羊和猴子的癌胚抗原（CEA）抗体进行免疫吸附纯化。从小鼠腹水中制备小鼠单克隆抗体（MAb）抗CEA I-38S1及其Fab片段。IgG制剂用123I或131I标记，Fab片段用131I标记。标记后抗体反应性未改变。晚期结直肠癌患者静脉注射50 - 200MBq 123I或30 - 160MBq 131I，其与250 - 500微克抗体或抗体片段偶联。使用发射断层扫描（SPECT）和/或传统γ相机闪烁扫描对患者进行检查。将标记的抗CEA在肿瘤中的特异性定位与通过CAT扫描、其他X射线方法或剖腹术确定的已知肿瘤进行比较，50%的已知肿瘤用绵羊抗CEA能准确定位。相比之下，70 - 80%的已知肿瘤部位用多克隆猴抗CEA抗体、单克隆抗CEA抗体或后者的Fab片段能正确定位。检测到一些先前未知的肿瘤。
