Larsson A, Ghosh R, Hammarström S
Department of Immunology, University of Stockholm, Sweden.
Cancer Immunol Immunother. 1989;30(2):92-6. doi: 10.1007/BF01665959.
To investigate whether anti-(carcinoembryonic antigen) monoclonal antibodies (mAb) react with single or repeated epitopes, sandwich radioimmunoassays in homologous and heterologous combinations were performed. Four mAb (I-27, I-47, II-17 and to some degree II-16) gave homologous binding while two mAb (I-38S1 and II-10) did not. Taken together with previous immunoprecipitation studies we conclude that all these mAb except II-10 react with repeated epitopes. The relative positions of the epitopes recognized by these mAb and of three additional mAb (II-6, II-7 and CB-CEA-1) were investigated using a plate antibody competition test with enzyme-labelled carcinoembryonic antigen (CEA). mAb I-38S1, II-6, II-7, II-10, II-16 and CB-CEA-1 were mutually cross-reactive, and were classified as belonging to one epitope group. mAb I-27 and I-47 fell outside this group and did not interfere with the binding of CEA conjugate to mAb II-17 either. They therefore represent a second epitope group. mAb II-17 showed no interference with the binding of CEA to any of the other mAb and must therefore represent a third epitope group. The slopes of the plate antibody competition curves were used for calculation of a correlation matrix, which in turn was used to depict the relative positions of the epitopes recognized by the mAb in the large group.
为研究抗癌胚抗原单克隆抗体（mAb）是与单个表位还是重复表位发生反应，进行了同源和异源组合的夹心放射免疫测定。四种单克隆抗体（I - 27、I - 47、II - 17以及在某种程度上II - 16）呈现同源结合，而两种单克隆抗体（I - 38S1和II - 10）则不然。结合先前的免疫沉淀研究，我们得出结论，除II - 10外，所有这些单克隆抗体均与重复表位发生反应。使用酶标记癌胚抗原（CEA）的平板抗体竞争试验研究了这些单克隆抗体以及另外三种单克隆抗体（II - 6、II - 7和CB - CEA - 1）所识别表位的相对位置。单克隆抗体I - 38S1、II - 6、II - 7、II - 10、II - 16和CB - CEA - 1相互交叉反应，被归为一个表位组。单克隆抗体I - 27和I - 47不属于该组，也不干扰CEA偶联物与单克隆抗体II - 17的结合。因此，它们代表第二个表位组。单克隆抗体II - 17不干扰CEA与任何其他单克隆抗体的结合，因此必定代表第三个表位组。平板抗体竞争曲线的斜率用于计算相关矩阵，进而用于描绘大组中各单克隆抗体所识别表位的相对位置。
