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介导的鼠李糖脂对乳腺癌MDA-MB-231细胞系的表征及细胞毒性

Characterization and Cytotoxicity of Mediated Rhamnolipids Against Breast Cancer MDA-MB-231 Cell Line.

作者信息

Mishra Neelam, Rana Kavita, Seelam Siva Deepthi, Kumar Rakesh, Pandey Vijyendra, Salimath Bharathi P, Agsar Dayanand

机构信息

Department of Microbiology, Gulbarga University, Gulbarga, India.

Department of Toxicology, Chaudhary Charan Singh University, Meerut, India.

出版信息

Front Bioeng Biotechnol. 2021 Nov 30;9:761266. doi: 10.3389/fbioe.2021.761266. eCollection 2021.

DOI:10.3389/fbioe.2021.761266
PMID:34950641
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8691732/
Abstract

A biosurfactant producing bacterium was identified as DNM50 based on molecular characterization (NCBI accession no. MK351591). Structural characterization using MALDI-TOF revealed the presence of 12 different congeners of rhamnolipid such as Rha-C8-C8:1, Rha-C10-C8:1, Rha-C10-C10, Rha-C10-C12:1, Rha-C16:1, Rha-C16, Rha-C17:1, Rha-Rha-C10:1-C10:1, Rha-Rha-C10-C12, Rha-Rha-C10-C8, Rha-Rha-C10-C8:1, and Rha-Rha-C8-C8. The radical scavenging activity of rhamnolipid (DNM50RL) was determined by 2, 3-diphenyl-1-picrylhydrazyl (DPPH) assay which showed an IC value of 101.8 μg/ ml. The cytotoxic activity was investigated against MDA-MB-231 breast cancer cell line by MTT (4,5-dimethylthiazol-2-yl-2,5-diphenyl tetrazolium bromide) assay which showed a very low IC50 of 0.05 μg/ ml at 72 h of treatment. Further, its activity was confirmed by resazurin and trypan blue assay with IC values of 0.01 μg/ml and 0.64 μg/ ml at 72 h of treatment, respectively. Thus, the DNM50RL would play a vital role in the treatment of breast cancer targeting inhibition of p38MAPK.

摘要

基于分子特征鉴定出一株产生物表面活性剂的细菌为DNM50（NCBI登录号：MK351591）。使用基质辅助激光解吸电离飞行时间质谱（MALDI - TOF）进行结构表征，结果显示存在12种不同的鼠李糖脂同系物，如鼠李糖 - C8 - C8:1、鼠李糖 - C10 - C8:1、鼠李糖 - C10 - C10、鼠李糖 - C10 - C12:1、鼠李糖 - C16:1、鼠李糖 - C16、鼠李糖 - C17:1、鼠李糖 - 鼠李糖 - C10:1 - C10:1、鼠李糖 - 鼠李糖 - C10 - C12、鼠李糖 - 鼠李糖 - C10 - C8、鼠李糖 - 鼠李糖 - C10 - C8:1和鼠李糖 - 鼠李糖 - C8 - C8。通过2,3 - 二苯基 - 1 - 苦基肼（DPPH）法测定鼠李糖脂（DNM50RL）的自由基清除活性，其IC值为101.8 μg/ml。采用MTT（4,5 - 二甲基噻唑 - 2 - 基 - 2,5 - 二苯基溴化四氮唑）法研究其对MDA - MB - 231乳腺癌细胞系的细胞毒性活性，在处理72小时时，其IC50极低，为0.05 μg/ml。此外，通过刃天青和台盼蓝试验进一步证实了其活性，在处理72小时时，IC值分别为0.01 μg/ml和0.64 μg/ml。因此，DNM50RL在靶向抑制p38丝裂原活化蛋白激酶（p38MAPK）治疗乳腺癌中可能发挥重要作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e836/8691732/628bafdbeb20/fbioe-09-761266-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e836/8691732/bd8a7600f7f9/fbioe-09-761266-g001.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e836/8691732/f262f320ecb8/fbioe-09-761266-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e836/8691732/011805ea9e1d/fbioe-09-761266-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e836/8691732/628bafdbeb20/fbioe-09-761266-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e836/8691732/bd8a7600f7f9/fbioe-09-761266-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e836/8691732/7b81464a4a86/fbioe-09-761266-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e836/8691732/c09e1b009080/fbioe-09-761266-g003.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e836/8691732/f262f320ecb8/fbioe-09-761266-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e836/8691732/011805ea9e1d/fbioe-09-761266-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e836/8691732/628bafdbeb20/fbioe-09-761266-g007.jpg

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