Li Jun, Ai Lianzhong, Xu Feiran, Hu Xintian, Yao Yijun, Wang Lifeng
College of Food Science and Engineering, Collaborative Innovation Center for Modern Grain Circulation and Safety, Key Laboratory of Grains and Oils Quality Control and Processing, Nanjing University of Finance and Economics, No. 3 Wenyuan Road, Nanjing, Jiangsu 210023, China.
School of Medical Instrument and Food Engineering, University of Shanghai for Science and Technology, Shanghai 200093, China.
Int J Biol Macromol. 2022 Feb 28;199:17-23. doi: 10.1016/j.ijbiomac.2021.12.089. Epub 2021 Dec 21.
In this study, an exopolysaccharide (EPS) was produced by Weissella cibaria NC516.11 isolated from distiller grains of Chinese Baijiu. The structural characterization of EPS determined using fourier transform infrared spectra and nuclear magnetic resonance spectra demonstrated that W. cibaria NC516.11 had α-(1 → 6) (93.46%) d-glucose linkages with a few α-(1 → 3) (6.54%) d-glucose linked branches. The monosaccharide composition of the EPS was glucose, and its molecular weight was 2.82 × 10 Da. Scanning electron microscopy showed that the microstructure of EPS had a three-dimensional structure at low magnification and a particle structure that protruded from the surface at high magnification. The addition of EPS into dough can promote the cross-linking of starch molecules and increase the water-holding capacity. Dynamic rheology indicated that the aqueous solution of EPS is a pseudoplastic fluid, and the higher the concentration of EPS, the greater the viscosity. The addition of EPS to the gluten-free dough showed G' > G″, which could increase the viscoelastic properties of the dough and enhance the gluten network.
在本研究中，从中国白酒酒糟中分离得到的魏斯氏菌（Weissella cibaria）NC516.11产生了一种胞外多糖（EPS）。利用傅里叶变换红外光谱和核磁共振光谱对EPS进行的结构表征表明，魏斯氏菌NC516.11具有α-(1→6)（93.46%）的d-葡萄糖连接，带有少量α-(1→3)（6.54%）的d-葡萄糖连接分支。EPS的单糖组成为葡萄糖，其分子量为2.82×10 Da。扫描电子显微镜显示，EPS的微观结构在低放大倍数下具有三维结构，在高放大倍数下具有从表面突出的颗粒结构。将EPS添加到面团中可促进淀粉分子的交联并增加持水能力。动态流变学表明，EPS水溶液是一种假塑性流体，EPS浓度越高，粘度越大。向无麸质面团中添加EPS显示G' > G″，这可以增加面团的粘弹性并增强面筋网络。
