Miao Miao, Dai Yongmei, Rui Can, Fan Yuru, Wang Xinyan, Fan Chong, Mu Juan, Hou Wenwen, Dong Zhiyong, Li Ping, Sun Guiju, Zeng Xin
Nanjing Maternity and Child Health Care Hospital, Women's Hospital of Nanjing Medical University, 210004, Nanjing, Jiangsu, P. R. China.
Key Laboratory of Environmental Medicine and Engineering of Ministry of Education, Department of Nutrition and Food Hygiene, School of Public Health, Southeast University, 210004, Nanjing, Jiangsu, P. R. China.
Diabetol Metab Syndr. 2021 Dec 24;13(1):150. doi: 10.1186/s13098-021-00768-8.
Gestational diabetes mellitus (GDM) has significant short and long-term health consequences for both the mother and child. There is limited but suggestive evidence that inulin could improve glucose tolerance during pregnancy. This study assessed the effect of inulin on glucose homeostasis and elucidated the molecular mechanisms underlying the inulin-induced antidiabetic effects during pregnancy.
Female C57BL/6 mice were randomized to receive either no treatment, high-dose inulin and low-dose inulin for 7 weeks with measurement of biochemical profiles. A real-time (RT) profiler polymerase chain reaction (PCR) array involved in glycolipid metabolism was measured.
Inulin treatment facilitated glucose homeostasis in a dose-dependent manner by decreasing fasting blood glucose, advanced glycation end products and total cholesterol, and improving glucose tolerance. Suppressing resistin (RETN) expression was observed in the inulin treatment group and the expression was significantly correlated with fasting blood glucose levels. The ratios of p-IRS to IRS and p-Akt to Akt in liver tissue and the ratio of p-Akt to Akt in adipose tissue as well as the expression level of GLUT4 increased significantly after inulin treatment.
Our findings indicated improvement of glucose and lipid metabolism by inulin was to activate glucose transport through the translocation of GLUT4 which was mediated by insulin signaling pathway repairment due to decreased expression of RETN and enhanced phosphorylation of IRS and Akt in GDM mice.
妊娠期糖尿病（GDM）对母亲和孩子均有显著的短期和长期健康影响。有有限但具有提示性的证据表明，菊粉可改善孕期的葡萄糖耐量。本研究评估了菊粉对葡萄糖稳态的影响，并阐明了孕期菊粉诱导抗糖尿病作用的分子机制。
将雌性C57BL/6小鼠随机分为三组，分别不接受治疗、接受高剂量菊粉和低剂量菊粉，为期7周，并测量生化指标。检测参与糖脂代谢的实时（RT） Profiler聚合酶链反应（PCR）阵列。
菊粉治疗通过降低空腹血糖、晚期糖基化终产物和总胆固醇，并改善葡萄糖耐量，以剂量依赖的方式促进葡萄糖稳态。在菊粉治疗组中观察到抵抗素（RETN）表达受到抑制，且该表达与空腹血糖水平显著相关。菊粉治疗后，肝脏组织中p-IRS与IRS的比值、p-Akt与Akt的比值以及脂肪组织中p-Akt与Akt的比值，以及GLUT4的表达水平均显著升高。
我们的研究结果表明，菊粉改善葡萄糖和脂质代谢的方式是通过GLUT4的易位激活葡萄糖转运，这是由GDM小鼠中RETN表达降低以及IRS和Akt磷酸化增强介导的胰岛素信号通路修复所介导的。
