Zhu Rining, Jiang Hexiang, Wang Jun, Bao Chuntong, Liu Haiyao, Li Fengyang, Lei Liancheng
College of Veterinary Medicine, Jilin University, Xi'an Road, Changchun, 5333, People's Republic of China.
College of Life Science and Engineering, Northwest Minzu University, Lanzhou, People's Republic of China.
AMB Express. 2021 Dec 24;11(1):175. doi: 10.1186/s13568-021-01336-z.
Porcine infectious pleuropneumonia is characterized by a high-rate of carriage and mixed infection with other pathogens. The host immune response induced by Actinobacillus pleuropneumoniae (APP) is the basis for elucidating pathogenesis and controlling disease. However, there is currently no comprehensive and dynamic data characterising the host immune response. In this study, piglets were infected with APP and differentially expressed proteins of bronchoalveolar lavage fluid (BALF) and peripheral serum were identified by iTRAQ-LC-MS/MS, and differentially expressed genes of peripheral blood mononuclear cells (PBMC) by RNA-seq. The results of the integrated analysis of serum, BALF and PBMC showed significant metabolism and local immune responses in BALF, the general immune response in PBMC mainly involves cytokines, while that in serum mainly involves biosynthesis, phagosome, and complement and coagulation cascades. Furthermore, immune responses in PBMCs and serum were rapid and maintained compared to the lung where metabolism and cell adhesion activities were enriched. Some innate immunity pathways of the cellular response to ROS, neutrophil mediated immunity, granulocyte activation and leukocyte cell-cell adhesion were identified as central points, connecting multiple signaling pathways to form an integrated large network. At 24 h post-infection, 14 molecules were up regulated in BALF, 10 of which were shared with PBMC, but at 120 h, 20 down-regulated molecules were identified in BALF, 11 of them still up- regulated in PBMC. We conclude that, the immune response in the lung is different from that in blood, but there is a similarity in response in PBMC and serum.
猪传染性胸膜肺炎的特点是携带率高且常与其他病原体混合感染。胸膜肺炎放线杆菌（APP）诱导的宿主免疫反应是阐明发病机制和控制疾病的基础。然而，目前尚无全面且动态的宿主免疫反应特征数据。在本研究中，仔猪感染APP后，通过iTRAQ-LC-MS/MS鉴定支气管肺泡灌洗液（BALF）和外周血清中的差异表达蛋白，通过RNA-seq鉴定外周血单核细胞（PBMC）中的差异表达基因。血清、BALF和PBMC的综合分析结果表明，BALF中存在显著的代谢和局部免疫反应，PBMC中的一般免疫反应主要涉及细胞因子，而血清中的免疫反应主要涉及生物合成、吞噬体以及补体和凝血级联反应。此外，与富含代谢和细胞黏附活性的肺相比，PBMC和血清中的免疫反应迅速且持续存在。细胞对ROS的反应、中性粒细胞介导的免疫、粒细胞激活和白细胞细胞间黏附等一些固有免疫途径被确定为关键点，连接多个信号通路形成一个综合的大网络。感染后24小时，BALF中有14个分子上调，其中10个与PBMC共享，但在120小时时，BALF中鉴定出20个下调分子，其中11个在PBMC中仍上调。我们得出结论，肺中的免疫反应与血液中的不同，但PBMC和血清中的反应存在相似性。
