Hesse J E, Lieber M R, Gellert M, Mizuuchi K
Cell. 1987 Jun 19;49(6):775-83. doi: 10.1016/0092-8674(87)90615-5.
Sequences encoding immunoglobulin variable domains are known to be assembled from variable (V), diversity (D), and joining (J) segments by site-specific recombination. We present a sensitive and rapid assay for V-(D)-J recombination that uses plasmid DNA transiently introduced into transformed pre-B cells, and demonstrates that the recombination is independent of any unique chromosomal context. Sequences sufficient to constitute recombination sites are contained within the 84 and 42 bp flanking, respectively, the murine J kappa 1 and V kappa L8 segments, which include the known heptamer-nonamer V-(D)-J joining signals. Deletion and inversion occur at comparable frequencies. Thus, V-(D)-J recombination may be relatively insensitive to the topological arrangement of sites, and events at the two novel junctions produced by the reaction may be coupled.
已知编码免疫球蛋白可变区的序列是通过位点特异性重组由可变（V）、多样（D）和连接（J）片段组装而成。我们提出了一种用于V-（D）-J重组的灵敏且快速的检测方法，该方法使用瞬时导入转化前B细胞的质粒DNA，并证明重组独立于任何独特的染色体环境。分别包含在小鼠Jκ1和VκL8片段侧翼84和42 bp内的序列足以构成重组位点，其中包括已知的七聚体-九聚体V-（D）-J连接信号。缺失和倒位以相当的频率发生。因此，V-（D）-J重组可能对位点的拓扑排列相对不敏感，并且反应产生的两个新连接点处的事件可能是相关联的。
