Hornebeck W, Soleilhac J M, Tixier J M, Moczar E, Robert L
Cell Biochem Funct. 1987 Apr;5(2):113-22. doi: 10.1002/cbf.290050206.
Rat leukocyte elastase has been purified successively by AH-Sepharose Kappa-elastin affinity chromatography and by ion exchange chromatography on a carboxymethyl Sephadex resin. It has great similarities with human leukocyte elastase in its molecular weight, substrate specificity and inhibitory profile. The effect of rat leukocyte elastase inhibitors in influencing the chemotactic response of rat PMN to fMetLeuPhe has been compared to that of other proteinase inhibitors. The results indicated that oleoyl (Ala)2ProValCH2Cl, a specific inhibitor of human and rat leukocyte elastases and Eglin C, which also inhibits human and rat cathepsin G, are among the powerful inhibitors of rat PMN chemotaxis induced by the formyl oligopeptide. This suggests that these neutral proteinases, in addition to their known participation in connective tissue catabolism, could play a role in PMN locomotion and chemotaxis.
大鼠白细胞弹性蛋白酶先后通过AH-琼脂糖κ-弹性蛋白亲和色谱法和羧甲基葡聚糖凝胶树脂离子交换色谱法进行了纯化。它在分子量、底物特异性和抑制谱方面与人类白细胞弹性蛋白酶有很大相似性。已将大鼠白细胞弹性蛋白酶抑制剂对大鼠中性粒细胞对fMetLeuPhe趋化反应的影响与其他蛋白酶抑制剂的影响进行了比较。结果表明，油酰基（丙氨酸）2脯氨酸缬氨酸CH2Cl，一种人和大鼠白细胞弹性蛋白酶的特异性抑制剂，以及也抑制人和大鼠组织蛋白酶G的埃格林C，是甲酰化寡肽诱导的大鼠中性粒细胞趋化作用的强效抑制剂之一。这表明这些中性蛋白酶，除了已知参与结缔组织分解代谢外，可能在中性粒细胞的运动和趋化作用中发挥作用。
