College of Animal Science and Technology, Northwest A&F University, Yangling 712100, China.
College of Animal Science and Technology, Henan University of Animal Husbandry and Economy, Zhengzhou 450046, China.
Comp Biochem Physiol B Biochem Mol Biol. 2022 Apr-May;259:110706. doi: 10.1016/j.cbpb.2021.110706. Epub 2021 Dec 23.
Our previous study has found that selenium (Se) can alleviate lipid accumulation caused by high-fat diet (HFD) in fish. This study aims to explore the selenoproteins (SePs) in grass carp Ctenopharyngodon idella by characterizing cDNAs of nine SeP genes (SELENOF, SELENOM, SELENOS, SELENOP1, SELENOP2, SELENOE, SELENOL, SELENOU1a and SELENOU1b) and measuring their transcriptional activity in response to HFD and HFD supplemented with 0.3 mg/Kg and 0.6 mg/Kg of Se (HSe 0.3 and HSe 0.6). Firstly, the nine SeP genes in grass carp encoded proteins with conserved functional protein regions in fish and other vertebrates. Secondly, the nine SeP genes except SELENOS showed high expression levels in the hepatopancreas, but in the adipose tissue, only SELENOS, SELENOE and SELENOU1b showed high expression levels. Further, HFD significantly up-regulated the expressions of SELENOF and SELENOS in the hepatopancreas and SELENOM in the adipose tissue of grass carp (P < 0.05), but significantly down-regulated the expressions of SELENOU1b in the hepatopancreas, SELENOP2, SELENOE, SELENOL and SELENOU1a in the adipose tissue and SELENOM in the muscle of grass carp (P < 0.05). In addition, for the hepatopancreas, the expressions of SELENOS in the HSe 0.3 group and SELENOF, SELENOM and SELENOP2 in the HSe 0.6 group significantly decreased compared with the HFD group (P < 0.05). For the adipose tissue, the expressions of SELENOF, SELENOP2, SELENOL, SELENOU1a and SELENOU1b in the HSe 0.3 group and SELENOP2, SELENOE, SELENOU1a and SELENOU1b in the HSe 0.6 group significantly increased compared with the HFD group (P < 0.05). In summary, the transcriptional activities of the nine SeP genes were regulated by the HFD and HFD supplemented with Se, indicating the potential role of these genes in the Se regulated lipid metabolism processes in grass carp, which is worthy of in-depth study.
我们之前的研究发现，硒（Se）可以减轻高脂肪饮食（HFD）引起的鱼类脂质积累。本研究旨在通过鉴定草鱼 Ctenopharyngodon idella 的 9 种硒蛋白（SeP）基因（SELENOF、SELENOM、SELENOS、SELENOP1、SELENOP2、SELENOE、SELENOL、SELENOU1a 和 SELENOU1b）的 cDNA，并测量它们对 HFD 和 HFD 补充 0.3 mg/Kg 和 0.6 mg/Kg 硒（HSe 0.3 和 HSe 0.6）的转录活性，来研究草鱼中的 SeP。首先，草鱼的 9 种 SeP 基因编码的蛋白质在鱼类和其他脊椎动物中具有保守的功能蛋白区域。其次，除 SELENOS 外，这 9 种 SeP 基因在肝胰腺中表达水平较高，但在脂肪组织中，只有 SELENOS、SELENOE 和 SELENOU1b 表达水平较高。此外，HFD 显著上调了草鱼肝胰腺中 SELENOF 和 SELENOS 以及脂肪组织中 SELENOM 的表达（P<0.05），但显著下调了肝胰腺中 SELENOU1b、脂肪组织中 SELENOP2、SELENOE、SELENOL 和 SELENOU1a 以及肌肉中 SELENOM 的表达（P<0.05）。此外，对于肝胰腺，HSe 0.3 组中 SELENOS 的表达和 HSe 0.6 组中 SELENOF、SELENOM 和 SELENOP2 的表达与 HFD 组相比显著降低（P<0.05）。对于脂肪组织，HSe 0.3 组中 SELENOF、SELENOP2、SELENOL、SELENOU1a 和 SELENOU1b 的表达以及 HSe 0.6 组中 SELENOP2、SELENOE、SELENOU1a 和 SELENOU1b 的表达与 HFD 组相比显著升高（P<0.05）。综上所述，这 9 种 SeP 基因的转录活性受 HFD 和 HFD 补充 Se 的调节，表明这些基因在草鱼 Se 调节的脂质代谢过程中可能发挥作用，值得深入研究。
