Key Laboratory of Tropical & Subtropical Fishery Resource Application & Cultivation, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510380, China.
Key Laboratory of Tropical & Subtropical Fishery Resource Application & Cultivation, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510380, China; Tianjin Key Lab of Aqua-Ecology and Aquaculture, Tianjin Agricultural University, Tianjin 300384, China.
Comp Biochem Physiol Part D Genomics Proteomics. 2020 Dec;36:100710. doi: 10.1016/j.cbd.2020.100710. Epub 2020 Jul 7.
Fat accumulation in the mesenteric adipose tissue is a serious problem in grass carp (Ctenopharyngodon idella) culture. Lipid droplet-related proteins (LDRPs) are involved in the formation, degradation, and biological functions of lipid droplets. In this study, we aimed to provide reference proteomics data to study lipid droplet regulation in fish. We isolated LDRPs from the mesenteric adipose tissue of grass carp (1-year-old) after normal feeding and 7 days of starvation, and identified and analysed them using isobaric tags for relative and absolute quantitation (iTRAQ) technology. Short-term starvation had no significant effect on the body weight, condition factor, visceral index, hepatopancreas index, intraperitoneal fat index, adipose tissue triglyceride content, and adipocyte size of grass carp. Nine hundred and fifty proteins were identified and annotated using the Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) databases; they are involved in a variety of metabolic and signalling pathways, including amino acid, lipid, and carbohydrate metabolism, and the PI3K-Akt signalling pathway. There were 296 differentially expressed proteins (DEPs), with 143 up-regulated and 153 down-regulated proteins. Three proteins involved in triglyceride and fatty acid syntheses and two proteins involved in autophagy were up-regulated, and six proteins involved in lipid catabolism were down-regulated. These results indicate that under short-term starvation, lipid droplets in the adipose tissue of grass carp may maintain their shape by promoting fat production and inhibiting lipolysis, and autophagy may be one of the main strategies for coping with short-term energy deprivation.
在草鱼（Ctenopharyngodon idella）养殖中，肠系膜脂肪组织中的脂肪积累是一个严重的问题。脂滴相关蛋白（LDRP）参与脂滴的形成、降解和生物学功能。在本研究中，我们旨在提供参考蛋白质组学数据来研究鱼类脂滴的调节。我们从正常饲养和 7 天饥饿后的草鱼（1 岁）肠系膜脂肪组织中分离 LDRP，并使用同位素标记相对和绝对定量（iTRAQ）技术对其进行鉴定和分析。短期饥饿对草鱼的体重、体况系数、内脏指数、肝胰脏指数、腹腔脂肪指数、脂肪组织甘油三酯含量和脂肪细胞大小没有显著影响。使用基因本体论（GO）和京都基因与基因组百科全书（KEGG）数据库鉴定和注释了 950 种蛋白质；它们涉及多种代谢和信号通路，包括氨基酸、脂质和碳水化合物代谢以及 PI3K-Akt 信号通路。有 296 种差异表达蛋白（DEP），其中 143 种上调，153 种下调。三种参与甘油三酯和脂肪酸合成的蛋白质和两种参与自噬的蛋白质上调，六种参与脂质分解代谢的蛋白质下调。这些结果表明，在短期饥饿下，草鱼脂肪组织中的脂滴可能通过促进脂肪生成和抑制脂肪分解来保持其形态，自噬可能是应对短期能量缺乏的主要策略之一。
