Savassi Bruna, Cordeiro Bárbara F, Silva Sara H, Oliveira Emiliano R, Belo Giovanna, Figueiroa Alessandra Gomes, Alves Queiroz Maria Izabel, Faria Ana Maria Caetano, Alves Juliana, da Silva Tales Fernando, Campos Gabriela Munis, Esmerino Erick A, Rocha Ramon S, Freitas Monica Q, Silva Marcia C, Cruz Adriano G, Vital Kátia Duarte, Fernandes Simone O A, Cardoso Valbert N, Acurcio Leonardo Borges, Jan Gwénaël, Le Loir Yves, Gala-Garcia Alfonso, do Carmo Fillipe Luiz R, Azevedo Vasco
Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, Brazil.
Faculdade de Veterinária, Universidade Federal Fluminense (UFF), Niterói, Brazil.
Front Pharmacol. 2021 Dec 10;12:755871. doi: 10.3389/fphar.2021.755871. eCollection 2021.
Mucositis is an adverse effect of cancer chemotherapies using 5-Fluorouracil (5-FU). It is characterized by mucosal inflammation, pain, diarrhea, and weight loss. Some studies reported promising healing effects of probiotic strains, when associated with prebiotics, as adjuvant treatment of mucositis. We developed a lyophilized symbiotic product, containing skimmed milk, supplemented with whey protein isolate (WPI) and with fructooligosaccharides (FOS), and fermented by BL23, B7, and B1. In a mice 5-FU mucositis model, this symbiotic lyophilized formulation was able to reduce weight loss and intestinal permeability. This last was determined by quantifying blood radioactivity after oral administration of 99mTc-DTPA. Finally, histological damages caused by 5-FU-induced mucositis were monitored. Consumption of the symbiotic formulation caused a reduced score of inflammation in the duodenum, ileum, and colon. In addition, it decreased levels of pro-inflammatory cytokines IL-1β, IL-6, IL-17, and TNF-α in the mice ileum. The symbiotic product developed in this work thus represents a promising adjuvant treatment of mucositis.
粘膜炎是使用5-氟尿嘧啶（5-FU）进行癌症化疗的一种不良反应。其特征为粘膜炎症、疼痛、腹泻和体重减轻。一些研究报告称，益生菌菌株与益生元联合使用时，作为粘膜炎的辅助治疗具有良好的愈合效果。我们开发了一种冻干共生产品，其包含脱脂牛奶，并添加了乳清蛋白分离物（WPI）和低聚果糖（FOS），并由BL23、B7和B1进行发酵。在小鼠5-FU粘膜炎模型中，这种共生冻干制剂能够减轻体重减轻和肠道通透性。后者是通过口服99mTc-DTPA后定量血液放射性来确定的。最后，监测了5-FU诱导的粘膜炎所造成的组织学损伤。食用共生制剂导致十二指肠、回肠和结肠的炎症评分降低。此外，它还降低了小鼠回肠中促炎细胞因子IL-1β、IL-6、IL-17和TNF-α的水平。因此，这项工作中开发的共生产品代表了一种有前景的粘膜炎辅助治疗方法。
