Merluzzi V J, Faanes R B, Czajkowski M, Last-Barney K, Harrison P C, Kahn J, Rothlein R
J Immunol. 1987 Jul 1;139(1):166-8.
Membrane-associated interleukin 1 (IL 1) activity was induced on the human macrophage tumor cell line, U937, by pretreatment with phorbol myristic acid (PMA). Incubation of PMA-treated, paraformaldehyde-fixed U937 cells with the murine cell line D10.G4.1 in the presence of concanavalin A caused an increase in DNA synthesis as measured by the uptake of tritiated thymidine. Paraformaldehyde-fixed U937, not pretreated with PMA, showed little or no activity. A rabbit polyclonal antibody directed against human IL 1 neutralized all membrane-associated IL 1-like activity, as measured by the inhibition of D10.G4.1 cell proliferation. PMA-treated U937 caused a pronounced enhancement of PGE2 production from a human chondrosarcoma cell line, SW-1353. Membrane-associated IL 1 induced a more potent PGE2 response than did a maximal concentration of soluble IL 1. Rabbit antihuman IL 1 neutralized membrane-bound IL 1 induction of PGE2. The data presented here raise the possibility that membrane-bound IL 1 may play a primary role in the pathophysiology of the inflammatory disease process.
用佛波肉豆蔻酸酯（PMA）预处理人巨噬细胞肿瘤细胞系U937，可诱导膜相关白细胞介素1（IL-1）活性。在伴刀豆球蛋白A存在的情况下，将经PMA处理并用多聚甲醛固定的U937细胞与鼠细胞系D10.G4.1共同孵育，通过氚标记胸腺嘧啶核苷的摄取来测量，结果显示DNA合成增加。未经PMA预处理的多聚甲醛固定的U937细胞几乎没有活性。一种针对人IL-1的兔多克隆抗体，通过抑制D10.G4.1细胞增殖来测量，可中和所有膜相关的IL-1样活性。经PMA处理的U937可显著增强人软骨肉瘤细胞系SW-1353中前列腺素E2（PGE2）的产生。与最大浓度的可溶性IL-1相比，膜相关IL-1诱导的PGE2反应更强。兔抗人IL-1可中和膜结合IL-1对PGE2的诱导作用。本文提供的数据增加了膜结合IL-1可能在炎症性疾病过程的病理生理学中起主要作用的可能性。
