Chen Lin, Yan Shuai, Yang Ming, Yu Fudong, Wang Jingjing, Wang Xiaoxin, Xu Huanbai, Shi Jianxia, Pan Ling, Zeng Yuexi, Li Siyu, Li Li, You Li, Peng Yongde
Department of Endocrinology and Metabolism, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine Shanghai 200080, China.
Basic Reproduction Laboratory, Shanghai Institute of Planned Parenthood Research, Fudan University Shanghai 200032, China.
Am J Transl Res. 2021 Nov 15;13(11):12601-12613. eCollection 2021.
The association of the gut microbiome with bone turnover markers (BTMs) in postmenopausal women is poorly understood.
Fecal samples were collected from 97 Chinese postmenopausal women, and the serum CTX and P1NP were determined. Individuals with serum CTX lower or higher than the median value were divided into LCTX and P1NP groups; and individuals with serum P1NP lower or higher than the median value were grouped into LP1NP and HP1NP groups. Microbiota profiles were determined by high-throughput 16S rRNA gene sequencing.
In postmenopausal women, only Faecalibacterium showed significant alteration in the HCTX group compared with the LCTX group (P=0.004, q=0.143). Linear discriminant analysis effect size (LEfSe) analysis revealed that Clostridiaceae (P=0.015, LDA=2.89), Faecalibacterium (P=0.017, LDA=4.60), Prevotella (P=0.040, LDA=3.61) and Clostridium (P=0.007, LDA=2.79) were abundant in the LCTX group, and Facklamia (P=0.044, LDA=3.10) was enriched in the HCTX group. Peptostreptococcaceae (P=0.048, LDA=2.83) and the SMB53 (P=0.028, LDA=2.05) genus were enriched in the LPINP group, and Veillonellaceae (P=0.025, LDA=4.43) and the S24_7 (P=0.023, LDA=3.08) family were enriched in the HPINP group. Six taxa correlated with BTMs in all subjects, including Clostridium (Clostridiaceae) that was negatively correlated with serum CTX amounts significantly (r=-0.34, P<0.001).
This study identified taxa-specific differences in the intestinal microflora associated with BTMs, notably CTX. These findings may help in uncovering the roles of gut microbiota on bone metabolism.
绝经后女性肠道微生物群与骨转换标志物（BTMs）之间的关联尚不清楚。
收集97名中国绝经后女性的粪便样本，并测定血清CTX和P1NP。血清CTX低于或高于中位数的个体分为LCTX组和HCTX组；血清P1NP低于或高于中位数的个体分为LP1NP组和HP1NP组。通过高通量16S rRNA基因测序确定微生物群谱。
在绝经后女性中，与LCTX组相比，HCTX组中只有粪杆菌显示出显著变化（P = 0.004，q = 0.143）。线性判别分析效应大小（LEfSe）分析显示，LCTX组中梭菌科（P = 0.015，LDA = 2.89）、粪杆菌（P = 0.017，LDA = 4.60）、普雷沃菌属（P = 0.040，LDA = 3.61）和梭菌属（P = 0.007，LDA = 2.79）丰富，而HCTX组中费克蓝姆菌属（P = 0.044，LDA = 3.10）富集。消化链球菌科（P = 0.048，LDA = 2.83）和SMB53属（P = 0.028，LDA = 2.05）在LPINP组中富集，韦荣球菌科（P = 0.025，LDA = 4.43）和S24_7科（P = 0.023，LDA = 3.08）在HPINP组中富集。在所有受试者中，有6个分类群与BTMs相关，包括梭菌属（梭菌科）与血清CTX含量呈显著负相关（r = -0.34，P < 0.001）。
本研究确定了与BTMs（尤其是CTX）相关的肠道微生物群中特定分类群的差异。这些发现可能有助于揭示肠道微生物群在骨代谢中的作用。
