Dept. of Agronomy, Purdue University, USA.
Dept. of Kinesiology, Pennsylvania State University, USA.
Food Funct. 2022 Nov 28;13(23):12316-12329. doi: 10.1039/d2fo02273g.
Prunes have health benefits, particularly in postmenopausal women. It is likely that the gut microbiome mediates some of these effects, but its exact role remains to be elucidated. This study aims to characterize the effect of prune supplementation on the gut microbiome of postmenopausal women. The fecal microbiome of 143 postmenopausal women ages 55-75 who met the compliance criteria in a randomized controlled trial of a 12-month dietary intervention in one of three treatment groups - no prunes ( = 52), 50 g prunes per day ( = 54), or 100 g prunes per day ( = 37) - was characterized at baseline and at the 12-month endpoint using 16S rRNA gene sequencing and QIIME2. Additional outcomes included assessment of select urinary phenolic metabolites and inflammatory markers. After 12 months, microbiomes of women consuming 50 g prunes had decreased evenness in bacteria taxa (Pielou's Evenness, Kruskal-Wallis = 0.026). Beta diversity comparisons indicated significant differences in microbiomes among prune treatments (Bray-Curtis PERMANOVA, = 0.005), and the effect was different at each prune dose ( = 0.057). Prunes enriched some bacterial taxa such as the family Lachnospiraceae (LEfSe LDA = 4.5). Some taxa correlated with urinary phenolic metabolites and inflammatory markers. negatively correlated with total urinary phenolics ( = -0.25, = 0.035) and Lachnospiraceae UCG-001 negatively correlated with plasma concentrations of IL-1β ( = -0.29, = 0.002). Differing gut microbiomes and correlation of some taxa with select phenolic metabolites and inflammatory markers, particularly Lachnospiraceae, after prune consumption suggest a potential mechanism mediating health effects. The microbiome differences at each dose may have implications for the use of prunes as a non-pharmacological whole food intervention for gut health.
李子具有健康益处，尤其是对绝经后妇女。肠道微生物群可能介导了其中的一些作用，但确切的作用仍有待阐明。本研究旨在描述李子补充对绝经后妇女肠道微生物群的影响。在一项为期 12 个月的饮食干预随机对照试验中，符合依从性标准的 143 名 55-75 岁绝经后妇女的粪便微生物群，被分为三组：不食用李子（=52）、每天食用 50 克李子（=54）或每天食用 100 克李子（=37）。在基线和 12 个月终点使用 16S rRNA 基因测序和 QIIME2 进行了特征描述。其他结果包括评估选定的尿酚类代谢物和炎症标志物。12 个月后，食用 50 克李子的女性的细菌分类群均匀度降低（皮埃罗均匀度，Kruskal-Wallis = 0.026）。β多样性比较表明，李子处理之间的微生物组存在显著差异（Bray-Curtis PERMANOVA，= 0.005），且每个李子剂量的效果不同（= 0.057）。李子富集了一些细菌分类群，如 Lachnospiraceae 家族（LEfSe LDA = 4.5）。一些分类群与尿酚类代谢物和炎症标志物相关。与总尿酚类呈负相关（= -0.25，= 0.035），Lachnospiraceae UCG-001 与 IL-1β 的血浆浓度呈负相关（= -0.29，= 0.002）。食用李子后，肠道微生物组的差异以及一些分类群与选定的酚类代谢物和炎症标志物的相关性，特别是 Lachnospiraceae，表明了一种潜在的介导健康效应的机制。每个剂量的微生物组差异可能对将李子作为一种非药物全食物干预措施用于肠道健康具有重要意义。
