Dehennin L, Nahoul K, Scholler R
J Steroid Biochem. 1987 Mar;26(3):337-43. doi: 10.1016/0022-4731(87)90098-7.
A highly specific technique based on gas chromatography-mass spectrometry associated with stable isotope dilution was applied to the analysis of follicular fluid aspirated from preovulatory follicles of women under ovarian stimulation prior to in vitro fertilization. Deoxycorticosterone, 21-hydroxypregnenolone and 11-deoxycortisol have been identified and quantified in the nanomolar concentration range. Significant positive correlations were found between these 21-hydroxy-steroids and their immediate precursors, thus indicating a probable common cellular origin. Corticosterone was tentatively identified and cortisol was evidenced at concentrations lower than peripheral plasma levels. The occurrence in human follicular fluid of cortisol, together with different high concentration intermediates, constitutes evidence for ovarian intra-follicular 21-hydroxylase activity, and probably also for 11 beta-hydroxylation enzyme activity.
一种基于气相色谱-质谱联用与稳定同位素稀释的高特异性技术被应用于对体外受精前接受卵巢刺激的女性排卵前卵泡抽吸的卵泡液进行分析。已鉴定并定量了脱氧皮质酮、21-羟孕烯醇酮和11-脱氧皮质醇，其浓度范围为纳摩尔级。发现这些21-羟基类固醇与其直接前体之间存在显著正相关，从而表明可能有共同的细胞来源。初步鉴定出皮质酮，且检测到皮质醇的浓度低于外周血浆水平。人卵泡液中存在皮质醇以及不同的高浓度中间体，这构成了卵巢卵泡内21-羟化酶活性的证据，可能也证明了11β-羟化酶活性的存在。
