Gomez-Sanchez C E, Foecking M F, Shackleton C H, Chavarri M R, Gomez-Sanchez E P
J Steroid Biochem. 1987 Jan;26(1):105-11. doi: 10.1016/0022-4731(87)90037-9.
Cortisol has been shown to be metabolized in the zona glomerulosa of the adrenal gland through the same pathway involving the cytochrome P-450, corticosterone methyl oxidase by which corticosterone is transformed to 18-hydroxycorticosterone and aldosterone. When cortisol is the precursor, 18-hydroxycortisol and 18-oxocortisol are formed. 18-Hydroxycortisol can also be made at a similar rate in the bovine zona fasciculata and reticularis as in the zona glomerulosa. We studied the possibility that the formation of 18-hydroxycortisol in the zona fasciculata and reticularis might be through a different pathway involving initial 18-hydroxylation of 11-deoxycortisol before 11 beta-hydroxylation. Rat adrenal capsules or cores were incubated with 10 micrograms of cortisol or 11-deoxycortisol and the formation of 18-hydroxycortisol was measured by radioimmunoassay. Both capsules and cores transformed 11-deoxycortisol to 18-hydroxycortisol, but cortisol was only transformed in the capsular portion. Sixty-two rat adrenals were incubated with 10 mg of 11-deoxycortisol and the putative steroid, 18-hydroxy-11-deoxycortisol, was purified by TLC and HPLC and subjected to gas chromatography mass spectrometry. The mass spectra indicated that the steroid isolated was indeed 18-hydroxy-11-deoxycortisol. The function of this steroid is still unknown.
已表明皮质醇在肾上腺球状带通过与细胞色素P - 450相关的相同途径进行代谢，该途径涉及皮质酮甲基氧化酶，通过此酶皮质酮转化为18 - 羟皮质酮和醛固酮。当皮质醇作为前体时，会形成18 - 羟皮质醇和18 - 氧代皮质醇。在牛的束状带和网状带中，18 - 羟皮质醇的生成速率与球状带相似。我们研究了束状带和网状带中18 - 羟皮质醇的形成可能是通过一条不同途径的可能性，该途径涉及在11β - 羟化之前先对11 - 脱氧皮质醇进行18 - 羟化。将大鼠肾上腺被膜或髓质与10微克皮质醇或11 - 脱氧皮质醇一起孵育，通过放射免疫测定法测量18 - 羟皮质醇的生成。被膜和髓质都将11 - 脱氧皮质醇转化为18 - 羟皮质醇，但皮质醇仅在被膜部分发生转化。将62个大鼠肾上腺与10毫克11 - 脱氧皮质醇一起孵育，通过薄层层析（TLC）和高效液相色谱（HPLC）纯化假定的类固醇18 - 羟 - 11 - 脱氧皮质醇，并进行气相色谱 - 质谱分析。质谱表明分离出的类固醇确实是18 - 羟 - 11 - 脱氧皮质醇。这种类固醇的功能仍然未知。
